Evidence map›Paper›PMID 41511593›Full record

ArticleMarine biotechnology (New York, N.Y.)2026

Whole-body RNA Sequencing Analysis of the Post-larval Reveals the Sex-biased Gene and MiRNA in the Prawn Macrobrachium rosenbergii.

Kun Xie, Yu-Meng Shi, Jin-Xia Yang, Jia-Yan Chen, Xu Huang, Xin-Yu Liu, Li-Ping Chen, Jie Chen, Chao-Ping Lu, Gao-Feng Qiu

Abstract read
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In one paragraph

Article in Marine biotechnology (New York, N.Y.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

The trial behind it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Kun XieKey Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Engineering Research Center of Aquaculture, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China.
Yu-Meng ShiKey Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Engineering Research Center of Aquaculture, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China.
Jin-Xia YangKey Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Engineering Research Center of Aquaculture, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China.
Jia-Yan ChenKey Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Engineering Research Center of Aquaculture, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China.
Xu HuangKey Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Engineering Research Center of Aquaculture, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China.
Xin-Yu LiuKey Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Engineering Research Center of Aquaculture, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China.
Li-Ping ChenKey Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Engineering Research Center of Aquaculture, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China.
Jie ChenKey Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Engineering Research Center of Aquaculture, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China.
Chao-Ping LuShanghai Hangao-Wuyue Agricultural Technology Co., Ltd, Shanghai, 201500, China.
Gao-Feng QiuKey Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Engineering Research Center of Aquaculture, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China. gfqiu@shou.edu.cn.

Funding

National Key R&D Program of China project number 2018YFD0900201Natural Science Foundation of China project number 32373125
6 · The paper itself

Abstract

To identify sex-biased gene and miRNA expression during the critical period of sex differentiation in the prawn Macrobrachium rosenbergii, we performed whole-body RNA sequencing of males and females at post-larval days 40 (PL40) and 70 (PL70). We obtained a total of 18,244 known and 7,063 novel mRNAs, along with 1,474 known and 1,049 novel miRNAs. A differential expression analysis revealed 1,323 sex-biased genes and 34 sex-biased miRNAs as well as seven miRNA-mRNA co-expression pairs, including two pairs associated with sex differentiation. The top 30 GO terms and KEGG pathways associated with sex-biased loci at PL40 and PL70 were evaluated. Four genes and three miRNAs with potential roles in sex differentiation were identified. Notably, through dual-luciferase reporter gene assays in 293T cells, we verified that the miRNAs let-7b and miR-9-5p regulate the expression of the dsx gene. Our research provides new insight into the sex differentiation mechanisms in M. rosenbergii.

Indexed as

MicroRNAsPalaemonidaeSex DifferentiationAnimalsFemaleGene Expression ProfilingHEK293 CellsMaleRNA, MessengerSequence Analysis, RNAMicroRNAsRNA, MessengerCritical period of sex differentiationMacrobrachium rosenbergiiSex-biased genes and miRNASex-differentiation related biological processes

Identifiers

PMID41511593

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.