Evidence map›Paper›PMID 41511387›Full record

ArticleeLife2026

Affinity-guided labeling reveals P2X7 nanoscale membrane redistribution during BV2 microglial activation.

Benoit Arnould, Adeline Martz, Pauline Belzanne, Francisco Andrés Peralta, Federico Cevoli, Volodya Hovhannisyan, Yannick Goumon, Eric Hosy, Alexandre Specht, Thomas Grutter

Abstract read
In one paragraph

Article in eLife, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Benoit ArnouldLaboratoire de Chémo-Biologie Synthétique et Thérapeutique (CBST) UMR 7199, équipe Ingénierie Canaux Ioniques, Centre National de la Recherche Scientifique, Université de Strasbourg, Faculté de Pharmacie, Illkirch, France.
Adeline Martz *Laboratoire de Chémo-Biologie Synthétique et Thérapeutique (CBST) UMR 7199, équipe Ingénierie Canaux Ioniques, Centre National de la Recherche Scientifique, Université de Strasbourg, Faculté de Pharmacie, Illkirch, France.ORCID https://orcid.org/0009-0008-4751-2587
Pauline Belzanne *Interdisciplinary Institute for Neuroscience, CNRS, Université de Bordeaux, Bordeaux, France.
Francisco Andrés PeraltaLaboratoire de Chémo-Biologie Synthétique et Thérapeutique (CBST) UMR 7199, équipe Ingénierie Canaux Ioniques, Centre National de la Recherche Scientifique, Université de Strasbourg, Faculté de Pharmacie, Illkirch, France.ORCID https://orcid.org/0000-0002-0727-1706
Federico CevoliLaboratoire de Chémo-Biologie Synthétique et Thérapeutique (CBST) UMR 7199, équipe Ingénierie Canaux Ioniques, Centre National de la Recherche Scientifique, Université de Strasbourg, Faculté de Pharmacie, Illkirch, France.
Volodya HovhannisyanCentre National de la Recherche Scientifique-Unité Propre de Recherche (CNRS-UPR) 3212, Institut des Neurosciences Cellulaires et Intégratives, Strasbourg, France.
Yannick GoumonCentre National de la Recherche Scientifique-Unité Propre de Recherche (CNRS-UPR) 3212, Institut des Neurosciences Cellulaires et Intégratives, Strasbourg, France.
Eric HosyInterdisciplinary Institute for Neuroscience, CNRS, Université de Bordeaux, Bordeaux, France.ORCID https://orcid.org/0000-0002-2479-5915
Alexandre SpechtLaboratoire de Chémo-Biologie Synthétique et Thérapeutique (CBST) UMR 7199, équipe NanoParticules Intelligentes, Centre National de la Recherche Scientifique, Université de Strasbourg, Strasbourg, France.
Thomas GrutterLaboratoire de Chémo-Biologie Synthétique et Thérapeutique (CBST) UMR 7199, équipe Ingénierie Canaux Ioniques, Centre National de la Recherche Scientifique, Université de Strasbourg, Faculté de Pharmacie, Illkirch, France.ORCID https://orcid.org/0000-0002-4351-9203

Funding

Agence Nationale de la Recherche ANR-20-CE14-0016-2Interdisciplinary Thematic Institute NeuroStra ANR-17-EURE-0022International Center for Frontier Research in Chemistry Labex CSC-TGR-18International Center for Frontier Research in Chemistry (Labex CSC-TGR-18) and Région Grand Est PhD grantMinistere de la Recherche PhD grantUniversity of Strasbourg Institute for Advanced Study USIAS 2019
6 · The paper itself

Abstract

ATP-gated purinergic P2X7 receptors are crucial ion channels involved in inflammation. They sense abnormal ATP release during stress or injury and are considered promising clinical targets for therapeutic intervention. However, despite their predominant expression in immune cells such as microglia, there is limited information on P2X7 membrane expression and regulation during inflammation at the single-molecule level, necessitating new labeling approaches to visualize P2X7 in native cells. Here, we present

Indexed as

Cell MembraneMicrogliaReceptors, Purinergic P2X7Staining and LabelingAdenosine TriphosphateAffinity LabelsAnimalsCell LineHumansInterleukin-1betaLipopolysaccharidesMiceAdenosine TriphosphateAffinity LabelsInterleukin-1betaLipopolysaccharidesReceptors, Purinergic P2X7biochemistrychemical biologyhumanhuman HEK293T cellsmousemouse BV2 cellsneuroscienceratrat P2X sequences

Identifiers

PMID41511387
PMCPMC12788799

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.