Evidence map›Paper›PMID 41511318›Full record

ArticleCells2025

Ovulatory Signal-Driven H3K4me3 and H3K27ac Remodeling in Mural Granulosa Cells Orchestrates Oocyte Maturation and Ovulation.

Furui Wang, Wenjing Wang, Shuai Zhang, Yinjuan Wang, Ruimen Zhang, Lei An, Jianhui Tian, Guangyin Xi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Furui WangCollege of Animal Science and Technology, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, China.ORCID 0009-0000-9498-5880
Wenjing WangCollege of Animal Science and Technology, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, China.
Shuai ZhangCollege of Animal Science and Technology, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, China.
Yinjuan WangCollege of Animal Science and Technology, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, China.
Ruimen ZhangCollege of Animal Science and Technology, Guangxi University, Nanning 530004, China.
Lei AnCollege of Animal Science and Technology, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, China.
Jianhui TianCollege of Animal Science and Technology, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, China.
Guangyin XiCollege of Animal Science and Technology, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, China.ORCID 0000-0001-5997-6540

Funding

Inner Mongolia Autonomous Region Science and Technology Innovation Major Demonstration Project No. 2025ZDSF0018Major Science and Technology Project of Xinjiang Autonomous Region 2023A02011National Natural Science Foundation No. 32202672Sci-Tech Innovation 2030 Agenda No. 2022ZD040130301Sci-Tech Innovation 2030 Agenda No. 2023ZD040750202
6 · The paper itself

Abstract

Ovulation and granulosa cell luteinization are induced by ovulatory signals, including luteinizing hormone (LH) and human chorionic gonadotropin (hCG). Histone modifications enable rapid, signal-responsive transcriptional reprogramming. However, the effects of LH/hCG-induced histone modification changes on the mural granulosa cells (MGCs) function remain to be fully elucidated. By mining public datasets we integrated transcriptomic and histone-modification profiles of MGCs across the ovulatory interval and tracked LH/hCG-driven gene expression at three time points (0, 4, and 12 h after-hCG). During oocyte maturation, the 4 h LH-surge constitutes a critical window for meiotic resumption, during which many genes display rapid transcriptional changes followed by a return to baseline levels. Early-response genes are enriched for cell locomotion, inflammatory responses, the activation of signaling pathways, and histone modifications. Furthermore, LH/hCG-induced transcriptome remodeling is highly correlated with dynamic gains or losses of H3K4me3 and H3K27ac. Notably, we discovered for the first time that H3K27ac marks super-enhancers (SEs) that regulate LH/hCG-induced transcriptional activation in MGCs. Finally, through complementary in vitro and in vivo pharmacological inhibition, we demonstrate that LH/hCG governs oocyte maturation and ovulation by reshaping the MGC transcriptome via H3K4me3- and H3K27ac-dependent chromatin remodeling. In summary, our study advances the understanding of how gonadotropins regulate MGC function and oocyte maturation through histone-modification-mediated transcriptional control.

Indexed as

Granulosa CellsHistonesOocytesOvulationAnimalsChorionic GonadotropinFemaleHumansLuteinizing HormoneMiceSignal TransductionSuper EnhancersChorionic Gonadotropinhistone H3 trimethyl Lys4HistonesLuteinizing HormoneH3K27acH3K4me3histone modificationluteinizing hormonemural granulosa cellsoocyte maturationovulation

Identifiers

PMID41511318
PMCPMC12785594

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.