Evidence map›Paper›PMID 41511315›Full record

ArticleCells2025

Hypoxia Affects Stem Cell Fate in Patient-Derived Ileum Enteroids in a HIF-1α-Dependent Manner.

Zina M Uckeley, Carmon Kee, Carlos Ramirez, Victoria Karaluz, Ashwini K Sharma, Josmar Polanco, Freddie D Ortiz Martinez, Christopher I Mederos, Sorin O Jacobs, Ingrid J Groose and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Zina M UckeleyDepartment of Molecular Genetics and Microbiology, College of Medicine, University of Florida, 1200 Newell Drive, Gainesville, FL 32610, USA.ORCID 0000-0001-9770-9873
Carmon KeeVirology, Department of Infectious Disease, University Hospital Heidelberg, Im Neuenheimer Feld 344, 69120 Heidelberg, Germany.
Carlos RamirezInstitute of Pharmacy and Molecular Biotechnology (IPMB), BioQuant-Center for Quantitative Biology, Heidelberg University, 60120 Heidelberg, Germany.
Victoria KaraluzDepartment of Molecular Genetics and Microbiology, College of Medicine, University of Florida, 1200 Newell Drive, Gainesville, FL 32610, USA.
Ashwini K SharmaInstitute of Pharmacy and Molecular Biotechnology (IPMB), BioQuant-Center for Quantitative Biology, Heidelberg University, 60120 Heidelberg, Germany.
Josmar PolancoDepartment of Molecular Genetics and Microbiology, College of Medicine, University of Florida, 1200 Newell Drive, Gainesville, FL 32610, USA.ORCID 0009-0008-4436-8516
Freddie D Ortiz MartinezDepartment of Molecular Genetics and Microbiology, College of Medicine, University of Florida, 1200 Newell Drive, Gainesville, FL 32610, USA.
Christopher I MederosDepartment of Molecular Genetics and Microbiology, College of Medicine, University of Florida, 1200 Newell Drive, Gainesville, FL 32610, USA.
Sorin O JacobsDepartment of Molecular Genetics and Microbiology, College of Medicine, University of Florida, 1200 Newell Drive, Gainesville, FL 32610, USA.ORCID 0000-0002-7868-0793
Ingrid J GrooseDepartment of Molecular Genetics and Microbiology, College of Medicine, University of Florida, 1200 Newell Drive, Gainesville, FL 32610, USA.
James M RamsdenDepartment of Molecular Genetics and Microbiology, College of Medicine, University of Florida, 1200 Newell Drive, Gainesville, FL 32610, USA.
Carl HerrmannInstitute of Pharmacy and Molecular Biotechnology (IPMB), BioQuant-Center for Quantitative Biology, Heidelberg University, 60120 Heidelberg, Germany.ORCID 0000-0003-4989-4722
Megan L StaniferDepartment of Molecular Genetics and Microbiology, College of Medicine, University of Florida, 1200 Newell Drive, Gainesville, FL 32610, USA.
Steeve BoulantDepartment of Molecular Genetics and Microbiology, College of Medicine, University of Florida, 1200 Newell Drive, Gainesville, FL 32610, USA.

Funding

BASIC MICROBIOLOGY &INFECTIOUS DISEASEST32AI007110 · NIAID · UNIVERSITY OF FLORIDA · PI Stephanie M Karst · 1985 to 2026
$4.3M
Influence of hypoxia on the antiviral functions of human intestinal epithelial cellsR01AI185510 · NIAID · UNIVERSITY OF FLORIDA · PI Steeve Boulant · 2025 to 2026
$1.3M
Deutsche Forschungsgemeinschaft 240245660Deutsche Forschungsgemeinschaft 272983813National Institute of Allergy and Infectious Diseases 1R01AI185510NIAID NIH HHS R01 AI185510NIAID NIH HHS T32 AI007110
6 · The paper itself

Abstract

The intestinal epithelium maintains tissue homeostasis through a dynamic balance of stem cell proliferation and differentiation. This process is spatially regulated along the crypt-villus axis, with intestinal stem cells in the crypt regions proliferating and progenitor cells differentiating as they migrate toward the villus tips. Because the lumen of the gut contains very low levels of oxygen (i.e., hypoxia), an oxygen gradient is established within the crypt-villus axis, placing the crypt regions under normoxic conditions while the villus tips reside under hypoxic conditions. Hence, intestinal epithelial cells encounter distinct oxygen microenvironments throughout their life span as they migrate along the crypt-villus structures during their proliferation and differentiation process. To investigate how oxygen availability influences intestinal stem cell proliferation and differentiation, we cultured patient-derived human ileum organoids (i.e., enteroids) under normoxic (20% oxygen) or hypoxic (1% oxygen) conditions. Under hypoxia, enteroid growth was reduced, and expression of several stem cell markers, such as

Indexed as

Hypoxia-Inducible Factor 1, alpha SubunitIleumOrganoidsStem CellsCell DifferentiationCell HypoxiaCell ProliferationHumansIntestinal MucosaWnt Signaling PathwayHIF1A protein, humanHypoxia-Inducible Factor 1, alpha Subunitenteroidsepitheliumhypoxiaileumintestinal stem cellsorganoid formation

Identifiers

PMID41511315
PMCPMC12785124

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.