ArticleArchives of insect biochemistry and physiology2026
Peptides Derived From Reactive Center Loops Inhibit Digestive Trypsin-Like Enzymes in Lepidopteran Pests.
Article in Archives of insect biochemistry and physiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- An Autolysis-Derived Trypsin Peptide Disrupts Digestive Homeostasis and Reduces Fitness inACS omega · 2026Article
- Structure-Guided Design of an Interface-Derived Inhibitor Peptide Against Spodoptera frugiperda Digestive Trypsins.Archives of insect biochemistry and physiology · 2026Article
- Synthetic Peptide Inhibition of Trypsin-Like Proteases in Spodoptera frugiperda (Lepidoptera: Noctuidae): Evaluating the Influence of Gut Microbiota.Archives of insect biochemistry and physiology · 2026Article
- Peptides Derived From Reactive Center Loops Inhibit Digestive Trypsin-Like Enzymes in Lepidopteran Pests.Archives of insect biochemistry and physiology · 2026Article
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Authors and funding
14 authors.
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Abstract
Soybean yield is often reduced by pest attacks. Among these, Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae) stands out as one of the most important defoliating pests of soybean. Therefore, the development of new bioinsecticides targeting Lepidopteran pests is an urgent need. Protease inhibitors (PIs) have emerged as promising molecules in this context. In this study, we designed four peptides (TGPCK, TGPCR, AVIMK, and AVIMR) inspired by the reactive center loops of BPTI and SKTI to assess their potential as competitive inhibitors of trypsin-like proteases in A. gemmatalis. In silico and kinetic analyses revealed that peptide binding affinity was influenced by specific chemical interactions, with pi-sigma bonds correlating with higher affinity for AVIMK, while alkyl/pi-alkyl and C-H bonds were associated with lower affinity for AVIMR and TGPCK. Key residues (His57, Asp102, Ser195, Asp189, S195, and G197) played a crucial role in ligand binding. Enzyme inhibition assays confirmed that all peptides acted as competitive inhibitors of A. gemmatalis trypsin-glen proteases, with TGPCK displaying the highest efficacy. These findings highlight BPTI-derived peptides as potential candidates for future pest management strategies. Further studies should evaluate their effects when applied to plants, considering possible metabolic interactions and phytotoxicity.
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