Evidence map›Paper›PMID 41510630›Full record

ArticleFEBS letters2026

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS.

Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P Damke, Stephen D Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P Vogel and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS letters, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Przemysław DutkaDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.ORCID https://orcid.org/0000-0003-3819-1618
Yuxi LiuDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Stefano MaggiDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Debnath GhosalDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Jue WangDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Prashant P DamkeDepartment of Veterinary Sciences, University of Kentucky College of Agriculture, Lexington, KY, USA.
Stephen D CarterDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Wei ZhaoDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Sukhithasri VijayrajratnamDepartment of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA.
Joseph P VogelDepartment of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA.
Carrie L ShafferDepartment of Veterinary Sciences, University of Kentucky College of Agriculture, Lexington, KY, USA.
Grant J JensenDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.

Funding

Structure and Function of Pathogenesis-Associated Bacterial Structures by Electron CryotomographyR01AI127401 · NIAID · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI GRANT J JENSEN · 2016 to 2026
$3.9M
National Institutes of Health (USA) R01-AI127401NIAID NIH HHS R01 AI127401
6 · The paper itself

Abstract

The Dot/Icm type IV secretion system (T4SS) is essential for Legionella pneumophila infection, but its in situ architecture and mechanism remain incompletely understood. Using cryo-electron tomography, we performed subtomogram averaging and 3D classification to resolve structural heterogeneity within the complex. We identified multiple assembly states of the inner membrane complex, including a fully assembled form with a hexamer-of-dimers DotO ATPase and symmetry mismatches between subcomplexes. A composite in situ model revealed a central channel above the inner membrane, likely used for substrate secretion. Imaging of infected macrophages showed T4SSs tethered to host vacuoles and extracellular vesicle release, suggesting additional effector delivery routes. These findings provide insight into Dot/Icm T4SS structure and infection-related dynamics.

Indexed as

Bacterial ProteinsLegionella pneumophilaType IV Secretion SystemsAnimalsCryoelectron MicroscopyMacrophagesModels, MolecularBacterial ProteinsType IV Secretion Systemsbacterial infectioncryo‐ETDot/Icm T4SSL. pneumophilasubtomogram averaging

Identifiers

PMID41510630
PMCPMC13244404

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.