Evidence map›Paper›PMID 41510448›Full record

ArticleCureus2025

Single-Cell Sequencing of a Bile Sample From an Acute Cholecystitis Patient.

Mari Tohya, Kazunori Murase, Masaaki Minagawa, Akio Saiura, Ichiro Nakagawa, Teruo Kirikae, Shin Watanabe

Abstract read
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In one paragraph

Article in Cureus, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Mari TohyaDivision of Biomedical Food Research, National Institute of Health Sciences, Kanagawa, JPN.
Kazunori MuraseDepartment of Microbiology, Graduate School of Medicine, Kyoto University, Kyoto, JPN.
Masaaki MinagawaDepartment of Hepatobiliary-Pancreatic Surgery, Graduate School of Medicine, Juntendo University, Tokyo, JPN.
Akio SaiuraDepartment of Hepatobiliary-Pancreatic Surgery, Graduate School of Medicine, Juntendo University, Tokyo, JPN.
Ichiro NakagawaDepartment of Microbiology, Graduate School of Medicine, Kyoto University, Kyoto, JPN.
Teruo KirikaeDepartment of Microbiome Research, Graduate School of Medicine, Juntendo University, Tokyo, JPN.
Shin WatanabeDepartment of Emergency Medicine, Graduate School of Medicine, Juntendo University, Tokyo, JPN.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single-cell sequencing is a novel approach to genome sequencing of clinical samples. However, there are only few studies using single-cell sequencing of genomes for bacterial infections. A 71-year-old woman presented to the emergency department with epigastric pain, 38.5°C fever, and a history of hypertension and hyperuricemia. From blood test results, acute cholecystitis was suspected. The surgery went well and bilirubin calcium stones were found in the gallbladder. Single-cell sequencing was used to investigate a bile sample from a patient with acute cholecystitis. The sample, cultured on a MacConkey agar plate, produced four colonies, all identified as

Indexed as

acute cholecystitise coli: escherichia colishigellasingle-cell sequencingwhole-genome sequencing

Identifiers

PMID41510448
PMCPMC12779362

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.