Evidence map›Paper›PMID 41510273›Full record

ArticleResearch square2025

Three-photon imaging of hippocampal neurogenesis through the intact mouse brain.

Yusaku Hontani, Xin Su, Yicheng Wu, Diana Machado, Megumi Mizoguchi, John Darby Cole, Hansjörg Kasper, Philipp Bethge, Fritjof Helmchen, Sebastian Jessberger

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yusaku HontaniLaboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland.ORCID 0000-0001-8853-9454
Xin SuLaboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland.
Yicheng WuLaboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland.
Diana MachadoLaboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland.
Megumi MizoguchiLaboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland.
John Darby ColeLaboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland.
Hansjörg KasperLaboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland.
Philipp BethgeLaboratory of Neural Circuit Dynamics, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland.
Fritjof HelmchenNeuroscience Center Zurich, University of Zurich and ETH Zurich, Zurich, Switzerland.ORCID 0000-0002-8867-9569
Sebastian JessbergerLaboratory of Neural Plasticity, Faculties of Medicine and Science, Brain Research Institute, University of Zurich, Zurich, Switzerland.ORCID 0000-0002-0056-8275

Funding

Dissemination of 3-photon imaging for chronic cellular imaging across speciesU01NS115585 · NINDS · ALLEN INSTITUTE · PI WATERS, JACK · 2020 to 2025
$2.9M
NINDS NIH HHS U01 NS115585
6 · The paper itself

Abstract

Multiphoton imaging allows for the visualization of structural and functional plasticity within the central nervous system. However, gaining optical access to deep brain structures, such as the hippocampal dentate gyrus (DG), requires invasive approaches, causing brain damage. Here we optimize three-photon (3P) microscopy to perform longitudinal imaging of the DG in the intact brain at unprecedented depth of up to 1800 μm. We apply this approach to follow the dynamics of neural stem cells (NSCs) in the adult and developing DG, allowing for novel insights into structural plasticity deep within the intact mouse brain.

Identifiers

PMID41510273
PMCPMC12776475

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.