ArticleIranian journal of microbiology2025
Molecular identification and diagnostic challenges of
Article in Iranian journal of microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background and Objectives: Materials and Methods: A one-month-old female neonate with suspected fungal sepsis yielded a yeast isolate. Initial identification was performed using API 20C AUX, followed by colony morphology assessment on Sabouraud Dextrose Agar. Molecular investigations included PCR amplification of the ITS1-5.8S rRNA-ITS2 region, restriction fragment length polymorphism (RFLP) with Results: The isolate was initially misidentified as Conclusion: This case emphasizes the limitations of conventional methods in identifying rare fungal pathogens, the critical role of molecular confirmation, and the importance of antifungal stewardship in guiding treatment. Reporting such cases contributes to global awareness, surveillance, and improved management of emerging yeast infections.
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