Evidence map›Paper›PMID 41509911›Full record

ArticleiScience2026

Membrane repair following filtroporation-induced cell permeabilization.

Isaura M Frost, Ruby Sims, Anya Yakimenko, Rachel Ma, Maximilian Floridia, Ruth A Foley, Emily C Duggan, Kelly Y Cai, Kelsey Jorgensen, Emily Skuratovsky and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Isaura M FrostDepartment of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Ruby SimsDepartment of Pediatrics, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Anya YakimenkoDepartment of Pediatrics, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Rachel MaDepartment of Pediatrics, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Maximilian FloridiaDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Ruth A FoleyDepartment of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Emily C DugganDepartment of Pediatrics, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Kelly Y CaiDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Kelsey JorgensenDepartment of Anthropology, University of Kansas, Lawrence, KS 66045, USA.
Emily SkuratovskyEli & Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Paul S WeissDepartment of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Steven J JonasDepartment of Pediatrics, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.

Funding

Nanoscience-Inspired Acoustofluidic Assembly Lines for Gene and Cellular TherapiesDP5OD028181 · OD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI JONAS, STEVEN JOHN · 2019 to 2023
$1.9M
NIH HHS DP5 OD028181
6 · The paper itself

Abstract

Mechanical disruption of cell membranes to enable intracellular delivery has been gaining traction as a methodology. Here, we show that a mechanical disruption-based strategy, filtroporation (FP), can be applied to edit the beta globin gene efficiently in human hematopoietic stem and progenitor cells. Gene expression analyses from RNA-Seq datasets demonstrate that electroporation (EP) yields greater transcriptional changes compared to FP globally, and gene pathway enrichment analyses suggest EP promotes stem cell differentiation while FP promotes cell division. Membrane repair occurs within 30 s of disruption by FP, and calcium signaling plays a key role in membrane repair in this context. Studies with fluorescently tagged membrane repair proteins, GRAF1, SNAP23, and CHMP4B, implicate the involvement of three mechanisms to reconstitute the cell barrier following FP. This work supports the evidence that the choice of intracellular delivery method affects transcriptional stem cell profile, and that membrane repair after mechanical disruption is a fast, multi-pathway process.

Indexed as

cell biologyomics

Identifiers

PMID41509911
PMCPMC12774684

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.