Evidence map›Paper›PMID 41509327›Full record

ArticlebioRxiv : the preprint server for biology2026

Unraveling Subcellular Ultrastructure with Cyclically Multiplexed Expansion Microscopy.

Seweryn Gałecki, Bo-Jui Chang, Felix Zhou, Qionghua Shen, Daniel Stoddard, Bingying Chen, Daniela Nicastro, Reto Fiolka, Kevin M Dean

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Seweryn GałeckiLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, TX 75390, USA.ORCID 0000-0002-7020-0804
Bo-Jui ChangLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, TX 75390, USA.ORCID 0000-0002-5513-7106
Felix ZhouLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, TX 75390, USA.ORCID 0000-0003-4463-1165
Qionghua ShenLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, TX 75390, USA.ORCID 0000-0002-2808-4408
Daniel StoddardDepartment of Biophysics, UT Southwestern Medical Center, Dallas, TX 75390, USA.
Bingying ChenLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, TX 75390, USA.ORCID 0000-0002-9590-3207
Daniela NicastroDepartment of Cell Biology, UT Southwestern Medical Center, Dallas, TX 75390, USA.ORCID 0000-0002-0122-7173
Reto FiolkaLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, TX 75390, USA.ORCID 0000-0002-4636-5000
Kevin M DeanLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, TX 75390, USA.ORCID 0000-0003-0839-2320

Funding

UT Southwestern Medical Center Simmons Comprehensive Cancer CenterP30CA142543 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI Kathryn Ann O'Donnell · 2010 to 2026
$53.7M
Technical Development Unit 2: Intelligent Hyperspectral Imaging of Subcellular Molecular States at the Whole Organ LevelU54CA268072 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI SEAN J MORRISON · 2021 to 2026
$9.1M
Structure and Function of Complexes that Regulate Ciliary MotilityR01GM083122 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI Daniela Nicastro · 2007 to 2026
$6.2M
UTSW-UNC Center for Cell Signaling AnalysisRM1GM145399 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI Kevin Michael Dean, Klaus M. Hahn · 2022 to 2026
$6.2M
Transformative microscopes to image across spatiotemporal scalesR35GM133522 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI Reto Paul Fiolka · 2019 to 2026
$3.1M
Omni Oblique Plane Microscope to spread light-sheet based imaging in biomedical researchR01EB035538 · NIBIB · UT SOUTHWESTERN MEDICAL CENTER · PI Reto Paul Fiolka · 2024 to 2026
$1.0M
Altering electron-induced radiolysis to optimize cryo-EM/ET imagingR01GM154131 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI NICASTRO, DANIELA · 2024 to 2025
$689k
Spinning Disk Confocal for UT SouthwesternS10OD028630 · OD · UT SOUTHWESTERN MEDICAL CENTER · PI LUBY-PHELPS, KATHERINE J · 2020 to 2020
$600k
NCI NIH HHS P30 CA142543NCI NIH HHS U54 CA268072NIBIB NIH HHS R01 EB035538NIGMS NIH HHS R01 GM083122NIGMS NIH HHS R01 GM154131NIGMS NIH HHS R35 GM133522NIGMS NIH HHS RM1 GM145399NIH HHS S10 OD028630
6 · The paper itself

Abstract

Despite advances in fluorescence microscopy, spectral overlap and limited resolution hinder the dense mapping of the cellular ultrastructure. To overcome these challenges, we developed Cy-ExM, a high-plex imaging strategy that integrates optimized cryo-fixation for antigen preservation, expansion microscopy, and iterative immunofluorescence labeling. Using oblique plane microscopy, we perform three-dimensional super-resolution imaging of 20 biological targets encompassing the full cellular volume of individual mammalian cells.

Identifiers

PMID41509327
PMCPMC12776423

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.