Evidence map›Paper›PMID 41506456›Full record

ArticleThe American journal of pathology2026

Keratocyte Depletion by Genetic Manipulation Re-creates Corneal Ectasia in a Mouse Model.

Ana C Acosta, Mei Sun, Isaac Poonen-Honig, Victoria Emerick, Sheila Adams, Marcel Y Avila, Curtis E Margo, Edgar M Espana

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The American journal of pathology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ana C AcostaCornea and External Disease, Department of Ophthalmology, Morsani College of Medicine, University of South Florida, Tampa, Florida.
Mei SunCornea and External Disease, Department of Ophthalmology, Morsani College of Medicine, University of South Florida, Tampa, Florida.
Isaac Poonen-HonigCornea and External Disease, Department of Ophthalmology, Morsani College of Medicine, University of South Florida, Tampa, Florida.
Victoria EmerickCornea and External Disease, Department of Ophthalmology, Morsani College of Medicine, University of South Florida, Tampa, Florida.
Sheila AdamsCornea and External Disease, Department of Ophthalmology, Morsani College of Medicine, University of South Florida, Tampa, Florida.
Marcel Y AvilaDepartment of Ophthalmology, Universidad Nacional de Colombia, Bogota, Colombia.
Curtis E MargoCornea and External Disease, Department of Ophthalmology, Morsani College of Medicine, University of South Florida, Tampa, Florida; Department of Pathology and Cellular Biology, Morsani College of Medicine, University of South Florida, Tampa, Florida.
Edgar M EspanaCornea and External Disease, Department of Ophthalmology, Morsani College of Medicine, University of South Florida, Tampa, Florida; Department of Molecular Pharmacology and Physiology, Morsani College of Medicine, University of South Florida, Tampa, Florida. Electronic address: eespana@usf.edu.

Funding

Regulation of tissue repair and scar formation by the stromal nicheR01EY034114 · NEI · UNIVERSITY OF SOUTH FLORIDA · PI Edgar Mauricio Espana · 2022 to 2026
$2.1M
Regulatory roles for collagen XII in establishment of corneal stromal structure and function and re-establishment after injuryR01EY029395 · NEI · UNIVERSITY OF SOUTH FLORIDA · PI ESPANA, EDGAR MAURICIO · 2018 to 2022
$1.8M
NEI NIH HHS R01 EY029395NEI NIH HHS R01 EY034114
6 · The paper itself

Abstract

Corneal ectasias are a significant cause of vision morbidity worldwide. In humans, corneal ectasias are characterized by tissue mechanical weakening, stromal thinning, and bulging. Previous histopathology studies showed a high rate of keratocyte apoptosis in corneas with ectasia. A mouse model expressing a keratocyte lineage-specific reporter KeraRT/tetO-Cre/mTmG/DTR was created to elucidate the roles of keratocyte death in the development of corneal ectasias. This mouse model allows selective death of keratocytes at chosen times during stromal development and in mature stromas. Slit-lamp examination as well as histopathology and advanced imaging techniques were used to assess stromal structure after keratocyte genetic ablation. It was found that genetic ablation of keratocytes in the first 20 days after birth induces corneal thinning and ectasia. A corneal hydrops-like phenotype (severe ectasia) occurred more frequently if keratocyte death was induced in the first week after birth. Inducing keratocyte death at an age where some degree of corneal maturation has occurred, >3 weeks of age, did not create changes in corneal thickness, transparency, or curvature, or any noticeable abnormalities in microstructure.

Indexed as

Corneal DiseasesCorneal KeratocytesAnimalsApoptosisCorneaCorneal StromaDilatation, PathologicDisease Models, AnimalMice

Identifiers

PMID41506456
PMCPMC13084596

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.