Evidence map›Paper›PMID 41506266›Full record

ArticleCell2026

In vivo detection of immune responses via cytokine activity labeling.

Guangqing Lu, Shanshan Zhang, Mengyang Feng, Eunha Kim, Daniel Cho, Jae Hyun Kim, Hannah Caris, Lev Silberstein, Gloria B Choi, Jun R Huh

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Guangqing LuDepartment of Immunology, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.
Shanshan ZhangDepartment of Immunology, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.
Mengyang FengThe Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, MA, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA.
Eunha KimBK21 Graduate Program, Department of Biomedical Sciences and Department of Neuroscience, Korea University College of Medicine, Seoul, Republic of Korea.
Daniel ChoThe Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, MA, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA.
Jae Hyun KimKosin University College of Medicine, Busan, Republic of Korea.
Hannah CarisThe Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, MA, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA.
Lev SilbersteinFred Hutchinson Cancer Research Center, Seattle, WA, USA.
Gloria B ChoiThe Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, MA, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA. Electronic address: gbchoi@mit.edu.
Jun R HuhDepartment of Immunology, Blavatnik Institute, Harvard Medical School, Boston, MA, USA; Bio2Q, Keio University, Tokyo, Japan. Electronic address: jun_huh@hms.harvard.edu.

Funding

Bacterial metabolites controlling Th17 cellsR01DK110559 · NIDDK · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI HUH, JUN R. · 2016 to 2024
$5.1M
Pregnancy influences maternal immune cell function and fetal brain developmentR01MH119459 · NIMH · HARVARD MEDICAL SCHOOL · PI Jun R. Huh · 2019 to 2026
$3.9M
Dissecting the modulatory functions of interleukin-17 in Alzheimer's DiseaseRF1AG080738 · NIA · HARVARD MEDICAL SCHOOL · PI HUH, JUN R. · 2023 to 2023
$2.4M
NIA NIH HHS RF1 AG080738NIDDK NIH HHS R01 DK110559NIMH NIH HHS R01 MH119459
6 · The paper itself

Abstract

While much is known about the identity and regulation of cytokine-producing cells, the cell types that respond to cytokines remain largely uncharacterized. To address this knowledge gap, we developed "cytokine cellular locating platforms" (CyCLoPs), a reporter system that translates cytokine receptor engagement into a genetically traceable signal. In vitro, CyCLoPs demonstrated high specificity, robust signal-to-background ratios, and broad applicability for probing diverse cytokine receptor interactions. In vivo, interleukin (IL)-17A-CyCLoPs reporter mice enabled the identification of IL-17A-responsive intestinal epithelial cells predominantly localized in the ileal villi following commensal bacterial colonization. Interferon-gamma (IFN-γ)-CyCLoPs reporter mice allowed for the detection of IFN-γ-exposed CD8

Indexed as

CytokinesAnimalsCD8-Positive T-LymphocytesEpithelial CellsHumansIleumInterferon-gammaInterleukin-17Intestinal MucosaMiceMice, Inbred C57BLReceptors, CytokineCytokinesInterferon-gammaInterleukin-17Receptors, CytokineCD8(+) T cellcytokine receptorIECIFN-γIL-17Aintestinal epithelial cell

Identifiers

PMID41506266
PMCPMC12961907

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.