Evidence map›Paper›PMID 41505254›Full record

ArticleCell reports2026

Cell-type-selective synaptogenesis during the development of layer 6 corticothalamic neuron connectivity in the mammalian neocortex.

Alan Y Gutman-Wei, Sriram Sudarsanam, Alec G Cabalinan, Naseer Shahid, Anny Shi, Luis E Guzman-Clavel, Sophia M Spindler-Krage, Amit Agarwal, Alex L Kolodkin, Solange P Brown

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Article in Cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Alan Y Gutman-WeiSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Sriram SudarsanamSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Alec G CabalinanSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Naseer ShahidSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Anny ShiSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Luis E Guzman-ClavelSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Sophia M Spindler-KrageSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Amit AgarwalThe Chica and Heinz Schaller Research Group, Institute for Anatomy and Cell Biology, Heidelberg University, Heidelberg, Germany; Interdisciplinary Center for Neurosciences, Heidelberg University, Heidelberg, Germany.
Alex L KolodkinSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Solange P BrownSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: spbrown@jhmi.edu.

Funding

Murine Mutagenesis CoreP30NS050274 · NINDS · JOHNS HOPKINS UNIVERSITY · PI KOLODKIN, ALEX L · 2005 to 2020
$10.9M
Training Program in NeuroscienceT32NS091018 · NINDS · JOHNS HOPKINS UNIVERSITY · PI JAMES J KNIERIM, Daniel Hans O'Connor · 2015 to 2026
$4.1M
Neuroscience Training ProgramT32EY017203 · NEI · JOHNS HOPKINS UNIVERSITY · PI BERGLES, DWIGHT E · 2005 to 2014
$2.9M
The Johns Hopkins Neuroscience Scholars Program (JHNSP)R25NS107167 · NINDS · JOHNS HOPKINS UNIVERSITY · PI BROWN, AMANDA MARIA · 2018 to 2022
$1.3M
The role of Poorly Characterized Disease-related Proteins in Cortical DevelopmentR03TR004616 · NCATS · JOHNS HOPKINS UNIVERSITY · PI KOLODKIN, ALEX L · 2023 to 2023
$164k
NCATS NIH HHS R03 TR004616NEI NIH HHS T32 EY017203NINDS NIH HHS P30 NS050274NINDS NIH HHS R25 NS107167NINDS NIH HHS T32 NS091018
6 · The paper itself

Abstract

The function of the mammalian neocortex relies on the timing of axon extension and establishment of cell-type-biased patterns of excitatory synaptic connections. A subtype of excitatory neurons, layer 6 corticothalamic neurons (L6CThNs), ultimately exhibits a marked preference for synapsing onto parvalbumin-positive (PV) inhibitory interneurons over more common excitatory cells in layers 6 and 4 (L6, L4). We show that the intracortical axons of L6CThNs develop in phases, first elongating within L6, then pausing before extending translaminar branches into L4. Decreasing L6CThN excitability selectively enhances axon growth in L6 but not the later elaboration in L4. For both layers, we test whether preferential synaptogenesis onto rarer PV interneurons, or non-selective synapse formation followed by selective pruning, generates adult connectivity. We find that L6CThNs form functional AMPA-receptor-containing synapses preferentially onto PV interneurons. Silent L6CThN synapses are not detected. Our findings show that cell-type-biased synaptogenesis underlies the formation of functional cell-type-specific excitatory connections in the neocortex.

Indexed as

NeocortexNeurogenesisNeuronsSynapsesThalamusAnimalsAxonsInterneuronsMiceNeurodevelopmentaxon developmentcortical circuitscorticothalamic neuronsCP: Developmental biologyCP: Neurosciencedendrite developmentKir2.1layer 6neocortexsilent synapsesomatosensory cortexsynaptogenesis

Identifiers

PMID41505254
PMCPMC12987384

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.