Evidence map›Paper›PMID 41504600›Full record

ReviewBiomacromolecules2026

Tunable Linkers for Dynamic Thiol-Based Bioconjugation Strategies.

G Cianfoni, L Pisano, D M Varouhaki, G Centioni, A Calcaterra, F Ghirga, C M Athanassopoulos, B Botta, S Cammarone, P Baiocco and 1 more

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Biomacromolecules, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

G CianfoniDepartment of Chemistry and Technology of Drugs, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.ORCID 0000-0002-6969-2071
L PisanoDepartment of Chemistry and Technology of Drugs, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.
D M VarouhakiDepartment of Chemistry and Technology of Drugs, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.
G CentioniDepartment of Chemistry and Technology of Drugs, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.
A CalcaterraDepartment of Chemistry and Technology of Drugs, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.ORCID 0000-0001-7036-6620
F GhirgaDepartment of Chemistry and Technology of Drugs, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.ORCID 0000-0002-5591-5190
C M AthanassopoulosDepartment of Chemistry, University of Patras, GR-26504 Rio-Patras, Greece.ORCID 0000-0002-7549-1911
B BottaDepartment of Chemistry and Technology of Drugs, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.ORCID 0000-0001-8707-4333
S CammaroneDepartment of Chemistry and Technology of Drugs, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.
P BaioccoDepartment of Biochemical Sciences "Alessandro Rossi Fanelli", Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy.
D QuaglioDepartment of Chemistry and Technology of Drugs, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.ORCID 0000-0001-7762-8283

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recent advancements in bioconjugation chemistry have increasingly focused on thiol-based strategies, offering reversible and stimuli-responsive mechanisms, particularly suited for biomedical applications. This review aims to provide a critical overview of the latest developments in thiol-containing linkers, such as disulfide bonds and photocleavable groups, emphasizing their role in enabling controllable and often reversible conjugation of biomolecules. The review will explore several applications, including peptide synthesis and peptide-stapling strategies, antibody-drug conjugates (ADCs), and responsive biomaterials, categorize key classes of cleavable thiol-based linkers, and analyze their mechanisms. Covering the literature from the past 15 years, focusing on innovations until 2024, this review addresses the chemical foundations and practical implementations of these systems, identifying current limitations and proposing future directions for designing selective, biocompatible, and functionally dynamic conjugation platforms.

Indexed as

Biocompatible MaterialsImmunoconjugatesPeptidesSulfhydryl CompoundsDisulfidesHumansBiocompatible MaterialsDisulfidesImmunoconjugatesPeptidesSulfhydryl Compounds

Identifiers

PMID41504600
PMCPMC12892336

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.