Evidence map›Paper›PMID 41503031›Full record

ArticleAmerican journal of ophthalmology case reports2026

Genome-sequencing of ocular fluid in a patient with syphilitic uveitis: Strain identification and antimicrobial resistance detection.

Nam V Nguyen, Jessica Wiley, Paul Garcia, Tolulope Fashina, Hythem Abouodah, Timothy Kaftan, Christopher D Conrady, Shaun T Cross, Michael R Wiley, Steven Yeh

Abstract readCase Reports
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of ophthalmology case reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Nam V NguyenDepartment of Ophthalmology, Stanley M. Truhlsen Eye Institute, University of Nebraska Medical Center, Omaha, NE, USA.
Jessica WileyPathoSeq Bio LLC, Omaha, NE, USA.
Paul GarciaDepartment of Ophthalmology, Stanley M. Truhlsen Eye Institute, University of Nebraska Medical Center, Omaha, NE, USA.
Tolulope FashinaDepartment of Ophthalmology, Stanley M. Truhlsen Eye Institute, University of Nebraska Medical Center, Omaha, NE, USA.
Hythem AbouodahDepartment of Ophthalmology, Stanley M. Truhlsen Eye Institute, University of Nebraska Medical Center, Omaha, NE, USA.
Timothy KaftanDepartment of Ophthalmology, Stanley M. Truhlsen Eye Institute, University of Nebraska Medical Center, Omaha, NE, USA.
Christopher D ConradyDepartment of Ophthalmology, Stanley M. Truhlsen Eye Institute, University of Nebraska Medical Center, Omaha, NE, USA.
Shaun T CrossDepartment of Environmental, Agricultural, and Occupational Health, College of Public Health, University of Nebraska Medical Center, Omaha, NE, USA.
Michael R WileyDepartment of Pathology, Microbiology, and Immunology, University of Nebraska Medical Center, Omaha, NE, USA.
Steven YehDepartment of Ophthalmology, Stanley M. Truhlsen Eye Institute, University of Nebraska Medical Center, Omaha, NE, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Purpose: To describe an unusual case of bilateral, hypertensive uveitis in which a syphilis genome with antimicrobial resistance was identified in the aqueous humor. Methods: A retrospective review of patient records was performed for clinical data. An aqueous humor sample was collected, and syphilis genomes were analyzed for phylogenetic traits and antimicrobial resistance. Results: A 64-year-old male was referred to our uveitis service for an evaluation of bilateral hypertensive anterior uveitis. On examination, visual acuities were 20/80 in the right eye (OD) and 20/100 in the left eye (OS), and intraocular pressure was 57 mmHg OD and 56 mmHg OS. Ophthalmic examination revealed severe conjunctival injection, severe anterior chamber inflammation, and vitreous opacities bilaterally. A uveitis laboratory work-up revealed a reactive syphilis IgG and an elevated RPR. Because of ongoing concern of a viral process, PCR testing was performed for HSV, VZV, and CMV, which were negative. Subsequent genomic sequencing of the residual ocular fluid demonstrated Conclusions and importance: This case report highlights the utility of ocular fluid's genome sequencing in diagnosing ocular syphilis and detecting antimicrobial resistance. Given the resurgence of syphilis in the U.S. and globally and the shortage of penicillin worldwide, genomic sequencing of ocular fluid may also assist in antimicrobial resistance detection for syphilis, along with pathogen identification, in concert with serologic assessment.

Indexed as

Antimicrobial resistanceGenome-sequencingMacrolide resistanceOcular fluidSyphilisSyphilitic uveitis

Identifiers

PMID41503031
PMCPMC12768858

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