ArticleACS omega2025
Gel Shrinkage in Discontinuous Electrophoresis: How to Stabilize the Electrolyte Boundary in EpitachophoresisPart 1Gel Selection.
Article in ACS omega, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gel electrophoresis is typically performed in a single electrolyte system. During the development of epitachophoresis for large-volume DNA concentration, which employs a discontinuous electrolyte system, we found that some gels tend to shrink significantly as the boundary between the leading electrolyte (LE) and trailing electrolyte (TE) moves along the gel. Effective stabilization of this boundary is crucial for analyte focusing, particularly in systems processing tens of milliliters of a sample. This study systematically evaluated various gel stabilization mediaincluding agarose-based gels (NEEO (no electroendosmosis), IsoGel, pulsed-field electrophoresis gel) and polyacrylamide gelsbased on their ability to maintain a stable LE/TE boundary, minimize gel shrinkage, and maximize DNA recovery. Agarose gels with low electroosmotic flow were optimized by adjusting the gel concentration, electrolyte composition, and pH and by incorporating Ba
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Registered trials
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