Evidence map›Paper›PMID 41502669›Full record

ArticleACS omega2025

Harnessing Contact-Quenched, Profluorescent Chemical Probes for Sensitive Determination and High-Throughput Measurements of Enzyme Activity.

Chien-Hui Huang, Chia-Yen Dai, Su-Hung Wang, Scott Severance, Chi-Ching Hwang, Yu-Chen Liu, Bao-Lin Yeh, Yung-Chieh Weng, Siao-Lei Yu, Hsing-Tao Kuo and 3 more

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Article in ACS omega, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Chien-Hui HuangDepartment of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
Chia-Yen DaiSchool of Medicine and Drug Development and Value Creation Research Centre, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
Su-Hung WangDivision of Hepato-Gastroenterology, Department of Internal Medicine, Chi Mei Hospital, Tainan 72263 Taiwan.
Scott SeveranceDepartment of Molecular and Cellular Sciences, Liberty University College of Osteopathic Medicine, Lynchburg, Virginia 24515, United States.
Chi-Ching HwangDepartment of Biochemistry, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
Yu-Chen LiuDepartment of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
Bao-Lin YehDepartment of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
Yung-Chieh WengDepartment of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
Siao-Lei YuDepartment of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
Hsing-Tao KuoDivision of Hepato-Gastroenterology, Department of Internal Medicine, Chi Mei Hospital, Tainan 72263 Taiwan.
Li-Fang WangDepartment of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
Jeh-Jeng WangDepartment of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.ORCID https://orcid.org/0000-0002-3148-1885
Tzu-Pin WangDepartment of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.ORCID https://orcid.org/0000-0003-3668-7329

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Dual-labeled, profluorescent chemical probes have been developed to quantify and visualize a specific enzyme's activity in complex biological media. The intact chemical probes often exhibit minimal fluorescence due to fluorescence quenching, but their intrinsic fluorescence can be released by a favorable enzyme-catalyzed reaction. Contact quenching represents one of several fluorescence quenching mechanisms. However, it is seldom intentionally implemented in the synthesis of dual-labeled, profluorescent chemical probes, because the structure of a contact quenching construct requires the formation of a ground-state fluorophore-quencher/-fluorophore complex. The ability of such dual-labeled molecular probes to act as intramolecular dimers cannot be predicted. We previously revealed that a

Identifiers

PMID41502669
PMCPMC12771411

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.