Evidence map›Paper›PMID 41502660›Full record

ArticleACS omega2025

Development of a Lateral Flow Immunoassay Strips for Rapid Antigen Detection of Dabie bandavirus.

Chao Huang, Bing-Yang Zheng, Zhi-Feng Li, Yuan-Fang Qin, Yong-Jun Jiao, Li Zhang, Lun-Biao Cui, Tian Wen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS omega, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Chao HuangNHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.ORCID https://orcid.org/0000-0002-4061-1573
Bing-Yang ZhengNHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.
Zhi-Feng LiNHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.
Yuan-Fang QinNHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.
Yong-Jun JiaoNHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.
Li ZhangNHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.
Lun-Biao CuiNHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.
Tian WenNHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study reports the development of a point-of-care (POC) serological assay for the detection of the Dabie bandavirus (DBV) infection. Specifically, this assay is based on the principle of lateral flow immunoassay (LFIA) strips. Two monoclonal antibodies against DBV were generated. They showed an optimal pairing. One mAb, named NP-10, was immobilized on the strip surface as the capture antibody. The other mAb, named NP-20, was labeled with colloidal gold nanoparticles (AuNPs) and used as the detector antibody. A series of parameters of this method were optimized during the AuNPs-mAb immune complex conjugating process, including the different pairing of antigens and antibodies, pH value, and the amount of coupled antibody. The whole detection process was completed within 15 min using 70 μL of the analyte solution, which consisted of 10 μL of sample and 60 μL of dilution buffer. The performance of the established assay was evaluated by using serum samples of DBV-infected individuals. It showed 90.9% clinical diagnostic sensitivity and 100% specificity with a κ coefficient of 0.929. The results demonstrated that our designed LFIA strips (LFIAs) used for DBV infection detection exhibited stable performance and reproducible outcomes. Consequently, this rapid and user-friendly LFIA can furnish physicians with preliminary test results. This enables them to make accurate diagnoses of DBV infections by integrating these results with other testing methods and clinical manifestations.

Identifiers

PMID41502660
PMCPMC12771437

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.