ArticleACS omega2025
Three-Dimensional Tumor Spheroids of Pancreatic and Colorectal Cancer Cell Lines for High-Throughput Screening of Drug Candidates.
Article in ACS omega, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed.
- METTL3-YTHDF1-driven mMolecular and cellular biochemistry · 2026Article
- Lactoferrin-Derived Peptides in Cancer Therapy: Structural Features, Mechanistic Insights and Clinical Translation Prospects.International journal of molecular sciences · 2026Review
- Investigation on the Cytotoxicity of Hydroxycinnamic and Hydroxybenzoic Acid-Based Natural Antioxidant Conjugated Terpyridine Analogues toward Triple-Negative Breast Cancer.ACS medicinal chemistry letters · 2026Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Over the past decade, three-dimensional (3D) cancer models have emerged as crucial tools in high-throughput screening (HTS), providing a more reliable bridge between in vitro and in vivo drug testing. These models demonstrate higher predictive accuracy compared to traditional two-dimensional (2D) cultures, although the lack of reproducibility and standardization is the main challenge for their translation into quantitative disease modeling and therapeutic response. Therefore, optimization and standardization of 3D spheroid cultivation protocols for HTS for various cancer cell types remain pertinent. This study establishes optimized protocols for cultivating four cancer cell lines (HCT116, SW620, PANC-1, and BxPc-3) as 3D spheroids for drug screening applications. Through systematic parameter optimization, we determined optimal seeding densities (500-1000 cells/well), formation times (3 days), and incubation periods (7-21 days) for each cell line. The application of both IC
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.