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ArticleClinical physiology and functional imaging2026

Intra- and inter-observer reliability of ultrasound muscle thickness of gluteal and biceps femoris long head in individuals with and without SCI.

Boas J Wijker, Sonja de Groot, Britt Denneman, Puck Brouwer, Vasileios Tomaras, Annelaura Haarler, Guido Weide, Lidwine B Mokkink, Johanna M van Dongen, Thomas W J Janssen

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Article in Clinical physiology and functional imaging, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Boas J WijkerDepartment of Human Movement Sciences, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands.ORCID https://orcid.org/0000-0001-7632-6515
Sonja de GrootDepartment of Human Movement Sciences, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands.
Britt DennemanDepartment of Human Movement Sciences, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands.
Puck BrouwerDepartment of Human Movement Sciences, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands.
Vasileios TomarasAmsterdam Rehabilitation Research Center, Reade, Amsterdam, the Netherlands.
Annelaura HaarlerDepartment of Human Movement Sciences, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands.
Guido WeideDepartment of Human Movement Sciences, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands.
Lidwine B MokkinkDepartment of Epidemiology and Data Science, Amsterdam UMC, Amsterdam, the Netherlands.
Johanna M van DongenDepartment of Health Sciences, Faculty of Science, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands.
Thomas W J JanssenDepartment of Human Movement Sciences, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands.

Funding

ZonMw 853001111
6 · The paper itself

Abstract

purposeThis study aimed to evaluate both inter- and intra-observer reliability of ultrasound-based muscle thickness measurements in able-bodied (AB) individuals, as well as intra-observer reliability in individuals with spinal cord injury (SCI).

methodsUltrasound measurements of the gluteus maximus, medius, minimus and biceps femoris long head were performed on 31 AB participants and 30 participants with SCI. Each AB participant was scanned on two occasions by three observers, with three repetitions per muscle per occasion. The muscle thickness in participants with SCI was measured using three repetitions during a single test occasion, conducted by one observer. A generalizability (G) study was conducted to assess the reliability of the measurements.

resultsIn AB participants, intra-observer reliability for gluteal muscles ranged from G-coefficient: 0.57 to 0.89, and for biceps femoris long head from G-coefficient: 0.60 to 0.76. Inter-observer reliability in AB participants was G-coefficient:0.48-0.72 for the gluteal muscles and G-coefficient: 0.52 for the biceps femoris. In contrast, intra-observer reliability in participants with SCI was excellent across all muscles (G-coefficient: 0.95-0.99).

conclusionUltrasound can assess muscle thickness with moderate to good intra-observer reliability in AB participants, but with only poor to moderate inter-observer reliability. In contrast, intra-observer reliability was excellent in participants with SCI. Reliability depends on observer experience and varies across muscles and populations.

Indexed as

Hamstring MusclesSpinal Cord InjuriesAdultButtocksCase-Control StudiesFemaleHumansMaleMiddle AgedMuscle, SkeletalObserver VariationPredictive Value of TestsReproducibility of ResultsUltrasonographyYoung Adultmeasurement reliabilitymeasurement reproducibilitymuscle morphologyrehabilitation imagingskeletal muscle assessmentsonography

Identifiers

PMID41502398
PMCPMC12780933

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