Evidence map›Paper›PMID 41501381›Full record

ArticleScientific reports2026

Lipidomic profiling of influenza A virus production in MDCK cells towards targeted clone selection.

Jocelyn A Menard, Tilia Zinnecker, Elena Godbout, Joshua A Roberts, Rozanne Arulanandam, Andrew Chen, Anne Landry, Christopher N Boddy, Udo Reichl, Jean-Simon Diallo and 2 more

Abstract read
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In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Jocelyn A Menard *Department of Chemistry, Carleton University, Ottawa, ON, Canada.
Tilia Zinnecker *Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.
Elena GodboutCentre for Cancer Therapeutics, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Joshua A RobertsDepartment of Chemistry, Carleton University, Ottawa, ON, Canada.
Rozanne ArulanandamCentre for Cancer Therapeutics, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Andrew ChenCentre for Cancer Therapeutics, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Anne LandryCentre for Cancer Therapeutics, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Christopher N BoddyDepartment of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON, Canada.
Udo ReichlMax Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.
Jean-Simon DialloCentre for Cancer Therapeutics, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Yvonne GenzelMax Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany. genzel@mpi-magdeburg.mpg.de.
Jeffrey C SmithDepartment of Chemistry, Carleton University, Ottawa, ON, Canada. JeffCSmith@CUNET.CARLETON.CA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

High-yield influenza virus production is essential for efficient vaccine manufacturing to support global demands. Using Madin-Darby canine kidney (MDCK) cells to produce influenza viruses is an attractive alternative to the conventional method of manufacturing vaccines using embryonated eggs. MDCK cells exhibit heterogeneity which can impact viral yields. However, the factors driving the variation between MDCK cells are not fully understood. Utilizing an untargeted liquid chromatography-mass spectrometry lipidomic approach, we investigated two proprietary MDCK clones (C59 and C113) provided by Sartorius (Germany) that differ in biochemical and viral production properties and examined their lipid profiles and dynamics upon influenza A virus (IAV) infection between 24 and 72 h. C113, a high-yield clone, displayed elevated levels across all lipid classes, aside from ether lipids compared to C59, a clone with superior growth properties. IAV infection in clone C59 and C113 displayed key differences, specifically triacylglycerols. Analysis of progeny virions from C59 and C113 clones revealed subtle differences with a positive correlation in lipid profile (R

Indexed as

Influenza A virusLipidomicsLipidsAnimalsChromatography, LiquidDogsMadin Darby Canine Kidney CellsVirus ReplicationLipids

Identifiers

PMID41501381
PMCPMC12783198

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.