Evidence map›Paper›PMID 41498970›Full record

ArticleNeurochemical research2026

UNC5B Promotes Post-Stroke Microglial Pyroptosis via DAPK3/MVK Pathway.

Ying Luo, Songjie Liao, Meiling Yao, Rui Wang, Jian Yu

Abstract read
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In one paragraph

Article in Neurochemical research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ying Luo *Department of Neurology, The First Affiliated Hospital, Sun Yat-Sen University; Guangdong Provincial Key Laboratory of Diagnosis and Treatment of Major Neurological Diseases; National Key Clinical Department and Key Discipline of Neurology, No. 58 Zhongshan Road 2, 510080, Guangzhou, China.
Songjie Liao *Department of Neurology, The First Affiliated Hospital, Sun Yat-Sen University; Guangdong Provincial Key Laboratory of Diagnosis and Treatment of Major Neurological Diseases; National Key Clinical Department and Key Discipline of Neurology, No. 58 Zhongshan Road 2, 510080, Guangzhou, China.
Meiling YaoDepartment of Neurology, The First Affiliated Hospital, Sun Yat-Sen University; Guangdong Provincial Key Laboratory of Diagnosis and Treatment of Major Neurological Diseases; National Key Clinical Department and Key Discipline of Neurology, No. 58 Zhongshan Road 2, 510080, Guangzhou, China.
Rui WangDepartment of Neurology, The First Affiliated Hospital, Sun Yat-Sen University; Guangdong Provincial Key Laboratory of Diagnosis and Treatment of Major Neurological Diseases; National Key Clinical Department and Key Discipline of Neurology, No. 58 Zhongshan Road 2, 510080, Guangzhou, China.
Jian YuDepartment of Neurology, The First Affiliated Hospital, Sun Yat-Sen University; Guangdong Provincial Key Laboratory of Diagnosis and Treatment of Major Neurological Diseases; National Key Clinical Department and Key Discipline of Neurology, No. 58 Zhongshan Road 2, 510080, Guangzhou, China. yujian@mail.sysu.edu.cn.

Funding

the National Natural Science Foundation of China 82071366the National Natural Scientific Foundation of Guangdong, China 2023A1515010486the Southern China International Cooperation Base for Early Intervention and Functional Rehabilitation of Neurological Diseases 2015B050501003
6 · The paper itself

Abstract

Pyroptotic inflammation has been shown to contribute to neuronal injury after stroke. Uncoordinated-5 homolog B (UNC5B) is implicated in neuroinflammation, and its downstream kinase death-associated protein kinase 3 (DAPK3) is predicted to interact with mevalonate kinase (MVK). To examine the role of UNC5B in post-stroke pyroptosis, we used a photothrombosis (PT) stroke model in mice and an oxygen-glucose deprivation (OGD) model in BV-2 microglia. Knockdown of Unc5b or Mvk and pharmacological inhibition of DAPK3 were performed, followed by detection of pyroptosis-associated proteins and cell viability. Interactions between DAPK3 and MVK were assessed using transwell coculture and co-immunoprecipitation. PT or OGD induced neuronal injury and increased expression of pyroptosis-related proteins. Knockdown of Unc5b or Mvk in microglia protected neurons by suppressing pyroptosis and disrupting the DAPK3-MVK protein complex. Upregulation of p-MVK was prevented by either Unc5b knockdown or DAPK3 inhibition, whereas DAPK3 upregulation was blocked only by Unc5b knockdown and not by Mvk knockdown. Our results suggest that UNC5B promotes post-stroke microglial pyroptosis in part through the DAPK3/MVK pathway.

Indexed as

Death-Associated Protein KinasesMicrogliaNetrin ReceptorsPyroptosisStrokeAnimalsMaleMiceMice, Inbred C57BLSignal TransductionDeath-Associated Protein KinasesNetrin ReceptorsDAPK3MVKPyroptosisStrokeUNC5B

Identifiers

PMID41498970

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.