Evidence map›Paper›PMID 41498795›Full record

ArticleMikrochimica acta2026

Ultrasensitive electrochemical sensor for HBV DNA detection based on exo III + HCR cascade amplification and label-assisted signal enhancement.

Jian Mao, Ju Hu, Haokang Du, Hongli Chen, Li Yang, Qinghua Yan

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jian MaoThe Key Laboratory of Biomedical Material, School of Life Science and Technology, Xinxiang Medical University, Xinxiang , 53003, China.
Ju HuThe Second Affiliated Hospital of Henan University of Science and Technology, Luoyang , 471000, PR China.
Haokang DuThe Key Laboratory of Biomedical Material, School of Life Science and Technology, Xinxiang Medical University, Xinxiang , 53003, China.
Hongli ChenThe Key Laboratory of Biomedical Material, School of Life Science and Technology, Xinxiang Medical University, Xinxiang , 53003, China.
Li YangCollege of Chemistry and Chemical Engineering, Henan Institute of Science and Technology, Xinxiang, 453003, China.
Qinghua YanThe Key Laboratory of Biomedical Material, School of Life Science and Technology, Xinxiang Medical University, Xinxiang , 53003, China. yqh3499@163.com.

Funding

Doctoral Scientific Research Foundation of Xinxiang Medical University 505304
6 · The paper itself

Abstract

Hepatitis B virus (HBV) infection is a leading cause of cirrhosis and hepatocellular carcinoma worldwide, and HBV DNA serves as a key molecular biomarker for diagnosis and therapeutic monitoring. Accurate and ultrasensitive quantification of HBV DNA is therefore crucial for early detection and clinical management. Herein, we developed an electrochemical DNA biosensor that integrates Exonuclease III (Exo III)-assisted target recycling with a hybridization chain reaction (HCR)-based cascade amplification. Platinum-decorated cobalt-copper carbon nanocubes (CoCu/CNC/Pt) nanozymes were employed as signal labels to synergistically amplify electrochemical responses, enabling highly sensitive detection of HBV DNA. Target DNA undergoes Exo III-catalyzed cyclic cleavage, releasing trigger strands (sDNA) that initiate the HCR process, driving the alternating hybridization of two hairpin probes (DNA1 and DNA2) into extended double-helical networks. These supramolecular structures present multiple terminal single-stranded regions that hybridize with complementary CoCu/CNC/Pt/aDNA probes, facilitating efficient anchoring of nanozyme labels on the electrode surface. The CoCu/CNC/Pt nanocomposites provide abundant redox-active sites and exhibit excellent catalytic activity toward H

Indexed as

Biosensing TechniquesDNA, ViralElectrochemical TechniquesExodeoxyribonucleasesHepatitis B virusCarbonCobaltCopperElectrodesHumansLimit of DetectionNanocompositesNucleic Acid Amplification TechniquesNucleic Acid HybridizationPlatinumCarbonCobaltCopperDNA, Viralexodeoxyribonuclease IIIExodeoxyribonucleasesPlatinumCascade amplificationChronoamperometryComposite nanomaterialElectrochemical DNA sensorHBV DNA detectionHybridization chain reaction

Identifiers

PMID41498795

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.