Evidence map›Paper›PMID 41497657›Full record

ArticlebioRxiv : the preprint server for biology2026

Fully computational design of PAM-relaxed

Youcai Xiong, Li-Kuang Tsai, Jun Zhou, Shuang Chen, Xiaofeng Xia, Jifeng Zhang, Y Eugene Chen, Jie Xu, Xiaoqiang Huang

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Youcai XiongCenter for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School, 2800 Plymouth Road, Ann Arbor, MI 48109, USA.
Li-Kuang TsaiCenter for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School, 2800 Plymouth Road, Ann Arbor, MI 48109, USA.
Jun ZhouCenter for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School, 2800 Plymouth Road, Ann Arbor, MI 48109, USA.
Shuang ChenCenter for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School, 2800 Plymouth Road, Ann Arbor, MI 48109, USA.
Xiaofeng XiaResearch & Development, ATGC Inc., 1004 W 9 Avenue, King of Prussia, PA 19406, USA.
Jifeng ZhangCenter for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School, 2800 Plymouth Road, Ann Arbor, MI 48109, USA.
Y Eugene ChenCenter for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School, 2800 Plymouth Road, Ann Arbor, MI 48109, USA.
Jie XuCenter for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School, 2800 Plymouth Road, Ann Arbor, MI 48109, USA.
Xiaoqiang HuangCenter for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical School, 2800 Plymouth Road, Ann Arbor, MI 48109, USA.ORCID 0000-0002-1005-848X

Funding

Novel methods to improve nuclease mediated homologous recombination, Administrative supplR42GM122181 · NIGMS · ATGC, INC. · PI ZHANG, JIFENG · 2022 to 2023
$2.1M
Structure-based computational engineering of saCas9 PAM requirementR41GM149016 · NIGMS · ATGC, INC. · PI HUANG, XIAOQIANG, XIA, XIAOFENG · 2023 to 2023
$275k
Development of miCas9 mediated gene editing therapies for cystic fibrosisR41HL164205 · NHLBI · GENETOBE INC. · PI XU, JIE · 2022 to 2022
$246k
NHLBI NIH HHS R41 HL164205NIGMS NIH HHS R41 GM149016NIGMS NIH HHS R42 GM122181
6 · The paper itself

Abstract

CRISPR-Cas9 nucleases have transformed genome engineering, yet their application is often constrained by protospacer-adjacent motif (PAM) requirements.

Identifiers

PMID41497657
PMCPMC12767333

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.