Evidence map›Paper›PMID 41497621›Full record

ArticlebioRxiv : the preprint server for biology2025

Overcoming the eIF2α Brake in Human Cell-Derived Translation Systems.

Nikolay A Aleksashin, Rohan R Shelke, Tianhao Yin, Jamie H D Cate

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Nikolay A AleksashinInnovative Genomics Institute, University of California, Berkeley, CA, USA.ORCID 0000-0002-3403-7587
Rohan R ShelkeDepartment of Molecular & Cell Biology, University of California, Berkeley, CA, USA.
Tianhao YinDepartment of Molecular & Cell Biology, University of California, Berkeley, CA, USA.
Jamie H D CateInnovative Genomics Institute, University of California, Berkeley, CA, USA.ORCID 0000-0001-5965-7902

Funding

Mechanisms of Translation Control in HumansR35GM148352 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI JAMIE H CATE · 2023 to 2026
$2.3M
NIGMS NIH HHS R35 GM148352
6 · The paper itself

Abstract

Cell-free translation from human cells is a powerful platform for studying mammalian gene expression and building synthetic biology tools, but productivity is often curtailed by inhibitory phosphorylation of eIF2α on residue Ser52. Here we systematically explored complementary strategies to bypass this initiation block across editable and hard-to-edit human cell types. In Expi293F suspension cells, precise genome editing of

Indexed as

cardiomyocytecell-free protein synthesiseIF2α phosphorylationExpi293FGADD34in vitro translationiPSCK3LPKRtranslation initiation controlWI-38

Identifiers

PMID41497621
PMCPMC12767633

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.