ArticleBeilstein journal of nanotechnology2025
Internal 3D temperature mapping in biological systems using ratiometric light-sheet imaging and lipid-coated upconversion nanothermometers.
Article in Beilstein journal of nanotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Upconversion nanoparticles (UCNPs) are well-known for their high efficiency, photostability, near-infrared excitation, and ability to estimate temperature through ratiometric imaging of two thermally coupled fluorescence bands. This work demonstrates the feasibility of volumetric temperature mapping in internal biological systems using light-sheet fluorescence microscopy and lipid-coated UCNPs as nanothermometry markers. This approach enables real-time thermal mapping with both high spatial and temporal resolution at the cellular and subcellular levels. To validate the method, we performed 3D temperature imaging on fixed
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