Evidence map›Paper›PMID 41496452›Full record

ArticlePlant communications2026

An all-in-one visual selection system for male-sterile line production in maize and rice.

Jinchu Liu, Zongkai Liu, Dahu Qin, Xiaolong Qi, Ming Chen, Dehe Cheng, Shuwei Guo, Xinyue Zhang, Liangliang Zhang, Tongzheng Yan and 12 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Jinchu LiuFrontiers Science Center for Molecular Design Breeding (MOE), National Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Zongkai LiuFrontiers Science Center for Molecular Design Breeding (MOE), National Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Dahu QinFrontiers Science Center for Molecular Design Breeding (MOE), National Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Xiaolong QiFrontiers Science Center for Molecular Design Breeding (MOE), National Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Ming ChenFrontiers Science Center for Molecular Design Breeding (MOE), National Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Dehe ChengFrontiers Science Center for Molecular Design Breeding (MOE), National Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Shuwei GuoFrontiers Science Center for Molecular Design Breeding (MOE), National Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Xinyue ZhangBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, China.
Liangliang ZhangFrontiers Science Center for Molecular Design Breeding (MOE), National Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Tongzheng YanFrontiers Science Center for Molecular Design Breeding (MOE), National Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Feifan LiFrontiers Science Center for Molecular Design Breeding (MOE), National Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Wenwen HeBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, China.
Zhixiao LuFrontiers Science Center for Molecular Design Breeding (MOE), National Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Hongshuo WangFrontiers Science Center for Molecular Design Breeding (MOE), National Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Junjun LiBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, China.
Xuhua YangBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, China.
Yunlu ShiCenter for Crop Functional Genomics and Molecular Breeding, China Agricultural University, Beijing 100193, China.
Shuaisong ZhangCenter for Crop Functional Genomics and Molecular Breeding, China Agricultural University, Beijing 100193, China.
Haiming ZhaoFrontiers Science Center for Molecular Design Breeding (MOE), National Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Chenxu LiuFrontiers Science Center for Molecular Design Breeding (MOE), National Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China. Electronic address: liucx@cau.edu.cn.
Shaojiang ChenFrontiers Science Center for Molecular Design Breeding (MOE), National Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China. Electronic address: chen368@126.com.
Yu ZhongFrontiers Science Center for Molecular Design Breeding (MOE), National Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, China. Electronic address: zhongyu306@cau.edu.cn.

Funding

Non-US Government Research Support type
6 · The paper itself

Abstract

Heterosis has significantly improved crop yields, yet hybrid seed production remains hindered by labor-intensive manual emasculation. Although current male-sterility systems, such as cytoplasmic male sterility and environment-sensitive genic male sterility, have improved the efficiency of hybrid seed production, their limited genetic adaptability and high environmental dependence remain major challenges. Here, we report an all-in-one seed production technology (ASPT) that integrates CRISPR-Cas9, RUBY, and key seed production technology (SPT) components into a single vector, enabling efficient generation and propagation of male-sterile lines in both maize and rice. The engineered RUBY marker enables visual identification of male-sterile and maintainer lines, with an accuracy of 99.81% in automated seed sorting and 100% in secondary field screening. Notably, ASPT was successfully introduced into 21 genetically diverse elite maize inbred lines, demonstrating broad compatibility. ASPT enables scalable and precise propagation of male-sterile lines in both maize and rice, providing a broadly applicable strategy to advance hybrid seed production in crops.

Indexed as

OryzaPlant BreedingPlant InfertilityZea maysCRISPR-Cas SystemsPlants, Genetically ModifiedSeedsASPTmaizemale-sterile linericeRUBY

Identifiers

PMID41496452
PMCPMC12903357

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.