Evidence map›Paper›PMID 41495911›Full record

ArticleNucleic acids research2026

ERK phosphorylates HNRNPF to promote the proliferation and suppress the differentiation of embryonic stem cells.

Xiaowei Duan, Yuying Wang, Jingai Zhang, Nannan Zhao, Qianyi Hao, Xiaoqiong Yang, Xiaoling Du, Hongjie Yang, Huabin Liang, Lingyi Chen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xiaowei DuanState Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Sciences, Frontiers Science Center for Cell Responses, National Demonstration Center for Experimental Biology Education and College of Life Sciences, Nankai University, Tianjin 300071, China.
Yuying WangState Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Sciences, Frontiers Science Center for Cell Responses, National Demonstration Center for Experimental Biology Education and College of Life Sciences, Nankai University, Tianjin 300071, China.
Jingai ZhangState Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Sciences, Frontiers Science Center for Cell Responses, National Demonstration Center for Experimental Biology Education and College of Life Sciences, Nankai University, Tianjin 300071, China.
Nannan ZhaoState Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Sciences, Frontiers Science Center for Cell Responses, National Demonstration Center for Experimental Biology Education and College of Life Sciences, Nankai University, Tianjin 300071, China.
Qianyi HaoState Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Sciences, Frontiers Science Center for Cell Responses, National Demonstration Center for Experimental Biology Education and College of Life Sciences, Nankai University, Tianjin 300071, China.
Xiaoqiong YangState Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Sciences, Frontiers Science Center for Cell Responses, National Demonstration Center for Experimental Biology Education and College of Life Sciences, Nankai University, Tianjin 300071, China.
Xiaoling DuState Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Sciences, Frontiers Science Center for Cell Responses, National Demonstration Center for Experimental Biology Education and College of Life Sciences, Nankai University, Tianjin 300071, China.
Hongjie YangDepartment of Colorectal Surgery, Tianjin Union Medical Center, The First Affiliated Hospital of Nankai University, Tianjin 300121, China.
Huabin LiangState Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Sciences, Frontiers Science Center for Cell Responses, National Demonstration Center for Experimental Biology Education and College of Life Sciences, Nankai University, Tianjin 300071, China.
Lingyi ChenState Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Sciences, Frontiers Science Center for Cell Responses, National Demonstration Center for Experimental Biology Education and College of Life Sciences, Nankai University, Tianjin 300071, China.ORCID 0000-0002-3695-3407

Funding

Foundation of the Committee on Science and Technology of Tianjin 24JCZDJC01340Foundation of the Committee on Science and Technology of Tianjin B08011National Key R&D Program of China 2021YFA1101002National Key R&D Program of China 2024YFA1108500
6 · The paper itself

Abstract

The mitogen-activated extracellular signal-regulated kinase/extracellular signal-regulated kinase (MEK/ERK) signaling plays a dual role in regulating the self-renewal and differentiation of embryonic stem cells (ESCs). How MEK/ERK promotes ESC self-renewal remains elusive, while the mechanisms for MEK/ERK to stimulate ESC differentiation have been investigated extensively. Here, we demonstrated that ERK2 phosphorylates heterogenous nuclear ribonucleoprotein F (HNRNPF) on Ser346 and Tyr356. Hnrnpf knockout reduces ESC proliferation rate, through downregulating CDK1 and CCNB1, and promotes ESC differentiation, attributed to reduced EED. Moreover, compared with the unphospho-mimetic mutant, the phospho-mimetic HNRNPF is more potent in rescuing ESC growth, as well as suppressing the mesodermal and trophectodermal differentiation. Mechanistically, HNRNPF binds to Cdk1, Ccnb1, and Eed messenger RNAs (mRNAs), thereby enhancing their translation efficiency. Additionally, phosphorylation of HNRNPF enhances its binding to these mRNAs and promotes their translation. Taken together, ERK may facilitate ESC proliferation and suppress lineage differentiation through phosphorylating HNRNPF.

Indexed as

Cell DifferentiationEmbryonic Stem CellsMitogen-Activated Protein Kinase 1AnimalsCDC2 Protein KinaseCell ProliferationCyclin B1HumansMAP Kinase Signaling SystemMicePhosphorylationRNA, MessengerCDC2 Protein KinaseCdk1 protein, mouseCyclin B1Mitogen-Activated Protein Kinase 1RNA, Messenger

Identifiers

PMID41495911
PMCPMC12774666

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.