Evidence map›Paper›PMID 41495644›Full record

ArticleBMC microbiology2026

Genomic characterization of Listeria monocytogenes isolated from small ruminants in integrated crop-livestock systems.

Sejin Cheong, Joanna G Rothwell, Cory Schlesener, Bart C Weimer, Craig Miramontes, Richard V Pereira, Paulo Pagliari, Alda F A Pires

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Sejin CheongDepartment of Population Health and Reproduction, School of Veterinary Medicine, University of California -Davis, Davis, CA, USA.
Joanna G RothwellDepartment of Population Health and Reproduction, School of Veterinary Medicine, University of California -Davis, Davis, CA, USA.
Cory SchlesenerDepartment of Population Health and Reproduction, School of Veterinary Medicine, University of California -Davis, Davis, CA, USA.
Bart C WeimerDepartment of Population Health and Reproduction, School of Veterinary Medicine, University of California -Davis, Davis, CA, USA.
Craig MiramontesDepartment of Population Health and Reproduction, School of Veterinary Medicine, University of California -Davis, Davis, CA, USA.
Richard V PereiraDepartment of Population Health and Reproduction, School of Veterinary Medicine, University of California -Davis, Davis, CA, USA.
Paulo PagliariDepartment of Soil, Water, and Climate, College of Food, Agriculture and Natural Resources Sciences, University of Minnesota, Saint Paul, MN, USA.
Alda F A PiresDepartment of Population Health and Reproduction, School of Veterinary Medicine, University of California -Davis, Davis, CA, USA. apires@ucdavis.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Listeria monocytogenes (L. monocytogenes), a foodborne pathogen shed by asymptomatic ruminants, poses a contamination risks in integrated crop-livestock systems (ICLS), where ruminants are introduced to graze cover crops prior to replanting fresh produce in a field. As a follow-up study, we conducted whole genome sequencing of 30 L. monocytogenes isolates obtained from sheep and goat fecal and soil samples collected during our previously published ICLS field trial studies (2019-2022) at organic farms in California and Minnesota. One goat fecal isolate was genetically identical to one soil isolate collected at seven days post-grazing. Most isolates (28/30, 93.3%) belonged to lineage I, specifically to serogroup IVb or IVb-v1, and were classified as CC1 or CC554, both clonal complexes previously associated with human listeriosis outbreaks. The majority of isolates harbored virulence associated genes, including LIPI-1 or LIPI-3 genes, and agrA, associated with biofilm formation and survival in agricultural soils. In antimicrobial susceptibility testing, all isolates were susceptible to antibiotics commonly used to treat ruminant and human listeriosis, including ampicillin and penicillin. These findings suggest that asymptomatic small ruminants in ICLS may introduce pathogenic L. monocytogenes strains into the agricultural environment, posing a potential contamination risk to fresh produce despite the low overall prevalence observed.

Indexed as

Listeria monocytogenesListeriosisAnimalsAnti-Bacterial AgentsCaliforniaCrops, AgriculturalFecesGenome, BacterialGenomicsGoatsLivestockMicrobial Sensitivity TestsMinnesotaSheepSoil MicrobiologyVirulence FactorsAnti-Bacterial AgentsVirulence FactorsAntimicrobial susceptibility testingIntegrated crop-livestock systemsListeria monocytogenesVirulence factorWhole-genome sequencing

Identifiers

PMID41495644
PMCPMC12870440

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.