Evidence map›Paper›PMID 41495425›Full record

ArticleMolecular human reproduction2026

Embryo secretome regulation of the endometrial epithelial transcriptome: development of a model to test for embryo viability.

Zhixing Jin, Yifan Wang, Abigail Freeman Blatchford, Helena Rodriguez-Caro, Luyao Wang, Christian M Becker, Pedro Melo, Tim Child, Rebecca Dragovic, Ingrid Granne and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular human reproduction, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Trial
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Zhixing JinNuffield Department of Women's and Reproductive Health, University of Oxford, Oxford, UK.
Yifan WangNuffield Department of Women's and Reproductive Health, University of Oxford, Oxford, UK.
Abigail Freeman BlatchfordNuffield Department of Women's and Reproductive Health, University of Oxford, Oxford, UK.
Helena Rodriguez-CaroNuffield Department of Women's and Reproductive Health, University of Oxford, Oxford, UK.ORCID 0000-0003-3907-9660
Luyao WangNuffield Department of Women's and Reproductive Health, University of Oxford, Oxford, UK.
Christian M BeckerNuffield Department of Women's and Reproductive Health, University of Oxford, Oxford, UK.ORCID 0000-0002-9870-9581
Pedro MeloNuffield Department of Women's and Reproductive Health, University of Oxford, Oxford, UK.
Tim ChildNuffield Department of Women's and Reproductive Health, University of Oxford, Oxford, UK.
Rebecca DragovicNuffield Department of Women's and Reproductive Health, University of Oxford, Oxford, UK.
Ingrid GranneNuffield Department of Women's and Reproductive Health, University of Oxford, Oxford, UK.ORCID 0000-0002-7056-5723
Jennifer H SouthcombeNuffield Department of Women's and Reproductive Health, University of Oxford, Oxford, UK.ORCID 0000-0003-0699-650X

Funding

Academy of Medical Sciences, Springboard SBF007\100078National Natural Science Foundation of China 82001523University of Oxford Medical Sciences Internal Fund 0014469
6 · The paper itself

Abstract

The aim of this study was to develop an advanced in vitro model of human endometrium using single-cell-derived endometrial epithelial organoids, enabling the study of embryo secretome-endometrial crosstalk at the maternal-fetal interface. Single-cell-derived organoids generated from endometrial tissue of a parous 39-year-old woman recapitulated hormone-responsive decidualization, as demonstrated by expression of SPP1 and acetyl-α-tubulin. When cultured in embryo culture media, organoid viability was maintained with no cytotoxicity, but proliferation was suppressed, likely due to the lower concentrations of the required factors in organoid growth media. Organoids were stimulated with culture supernatants from morphologically good-quality embryos with known pregnancy outcomes (live birth (n = 4) vs no pregnancy (n = 4)). Transcriptomic profiling (RNA-sequencing) revealed that 32 genes were differentially expressed (DEGs) in organoids exposed to the culture supernatants from live-birth embryos versus non-pregnant outcomes: 24 upregulated and 8 downregulated. These DEGs were enriched for biological processes related to cell motility and cytoskeletal dynamics. In conclusion, soluble factors secreted by human blastocysts achieving live birth selectively modulate the endometrial epithelial transcriptome, enhancing pathways involved in cytoskeletal remodeling and immune modulation. This embryo-directed remodeling likely facilitates endometrial receptivity for successful implantation to occur. Our organoid model provides a robust platform for further investigating implantation failure.

Indexed as

Embryo, MammalianEndometriumSecretomeTranscriptomeAdultBlastocystEmbryo ImplantationEpithelial CellsFemaleGene Expression ProfilingHumansOrganoidsPregnancyembryo culture supernatantembryo–endometrial dialogueendometrial epithelial organoidsin vitro modeltranscriptome

Identifiers

PMID41495425
PMCPMC12957937

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.