ArticleScientific reports2026
Sustainable HPLC method for simultaneous determination of Cefazolin, Sulfadimidine, and Marbofloxacin residues in milk.
Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Green HPTLC-densitometry for simultaneous determination of antibiotic residues in milk with greenness assessment and application to real samples.Scientific reports · 2026Article
- Veterinary Drug Residues in Food Chains: Sources, Exposure Pathways, Health Impacts, Mitigation, and Safety Assurance.Foods (Basel, Switzerland) · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antibiotic residues in milk pose a food safety risk by promoting antimicrobial resistance and causing toxic effects in consumers. This study developed and optimized a sensitive chromatographic method for the simultaneous quantification of Cefazolin (CFZ), Sulfadimidine (SDD), and Marbofloxacin (MFC) residues in milk. These antibiotics are commonly used as a combination therapy for managing cattle metritis. Separation was performed on a C18 column with UV detection at 270 nm. The eluent consisted of a 0.05 M phosphate buffer (pH 3), acetonitrile, and methanol in a 60:35:5 ratio, flowing at 1.0 mL/min. Sample preparation included protein precipitation with acetonitrile, followed by dispersive solid-phase extraction (dSPE) using Enhanced Matrix Removal-Lipid (EMR-L) tubes to remove interfering lipids. Validation per ICH-Q2R1 showed a linearity range of 0.0125–0.5 µg/mL for CFZ and MFC and 0.025–0.5 µg/mL for SDD, with LOD/LOQ values between 0.01 and 0.015 µg/mL and % recoveries between 97.67% and 98.89%. The method effectively quantified the studied drug residues in real cattle milk samples after their withdrawal period, with no significant differences in accuracy or precision versus established methods. Additionally, an assessment of the method’s sustainability (Eco-scale: 71, AGREE: 0.69, RGB12: 88.8) confirmed its compliance with green and white analytical chemistry principles.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.