Evidence map›Paper›PMID 41493529›Full record

ArticleWorld journal of microbiology & biotechnology2026

DpAGPs overexpression enhanced application value of Dunaliella Parva for bioethanol production.

Limei Huang, Jin Zhang, Yuwen Guo, Shiyu Liang, Changhua Shang

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Article in World journal of microbiology & biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Limei HuangKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education & Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University) & University Engineering Research Center of Bioinformation and Genetic Improvement of Specialty Crops, Guilin, Guangxi, 541006, China.
Jin ZhangKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education & Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University) & University Engineering Research Center of Bioinformation and Genetic Improvement of Specialty Crops, Guilin, Guangxi, 541006, China.
Yuwen GuoKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education & Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University) & University Engineering Research Center of Bioinformation and Genetic Improvement of Specialty Crops, Guilin, Guangxi, 541006, China.
Shiyu LiangKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education & Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University) & University Engineering Research Center of Bioinformation and Genetic Improvement of Specialty Crops, Guilin, Guangxi, 541006, China.
Changhua ShangKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education & Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University) & University Engineering Research Center of Bioinformation and Genetic Improvement of Specialty Crops, Guilin, Guangxi, 541006, China. shangchanghua@mailbox.gxnu.edu.cn.

Funding

2024 Joint Fund for Guangxi Normal University and National Natural Science Foundation of China 2024PY020Guangxi Key Research and Development Program 2023AB01132Natural Science Foundation of Guangxi Zhuang Autonomous Region 2024JJA130020
6 · The paper itself

Abstract

Dunaliella parva is halophilic green alga. Dunaliella parva may produce a large amount of starch, which has great practical value for bioethanol production. Glucose may be catalyzed by rate-limiting enzyme ADP-glucose pyrophosphorylase to produce starch. Our previous study cloned DpAGPs gene encoding ADP-glucose pyrophosphorylase (AGPase) small subunit from Dunaliella parva (D. parva). However, the function of DpAGPs remains unclear at present. To assess the influences of DpAGPs on metabolism of D. parva, this paper constructed recombinant plasmid pBI221-UbiΩ-CAT-AGPs, and transformed recombinant plasmid and empty plasmid pBI221-GFP-UbiΩ-CAT to D. parva. In transgenic D. parva overexpressing DpAGPs (sample D.parva T), both protein, carbohydrate and starch contents, AGPase activity and DpAGPs mRNA level increased compared with control sample D.parva C overexpressing the empty plasmid. Protein content of D.parva T (88.38 mg/g dry weight) increased by 11.18 mg/g DW compared with D.parva C (77.20 mg/g dry weight) at 15 d. In particular, compared with sample D.parva C, starch contents sharply increased by 34.83 mg/g dry weight (DW) and 53.51 mg/g DW, and carbohydrate contents sharply increased by 143.24 mg/g DW and 143.66 mg/g DW at 15 d/18 d in DpAGPs transgenic D.parva T. However, pigments (chlorophyll a, chlorophyll b and carotenoid) and oil contents decreased in D.parva T. The absolute oil contents of D.parva T (23.97% and 21.79%) decreased by 25.40% and 27.68% compared with D.parva C (49.37% and 49.47%) at 15 d and 18 d, respectively. This paper observed the role of DpAGPs in D. parva, providing a reference for improving microalgal starch content and enhancing the practical value of D. parva for bioethanol production. The engineered D. parva with higher starch and carbohydrate contents had a great potential for bioethanol production.

Indexed as

BiofuelsChlorophyceaeEthanolGlucose-1-Phosphate AdenylyltransferaseFermentationGlucosePlasmidsStarchBiofuelsEthanolGlucoseGlucose-1-Phosphate AdenylyltransferaseStarchBioethanol productionDpAGPs geneDunaliella parvaOverexpressionStarch content

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.