Evidence map›Paper›PMID 41492495›Full record

ArticleMatrix biology plus2026

P-LM421E8, the heparan sulfate chain-conjugated laminin-421-E8 fragment, drives differentiation of human induced pluripotent stem cells into hematopoietic progenitor cells comparable to basic fibroblast growth factor in a chemically defined system.

Naoto Ninomiya, Kaoru Sasaki, Ryosuke Katori, Yasuhiro Shimizu, Kazumasa Fujita, Yukimasa Taniguchi, Taiko Kunieda, Kouichi Tamura, Masashi Yamada, Kiyotoshi Sekiguchi and 1 more

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Article in Matrix biology plus, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Naoto NinomiyaResearch Division, Kobe Research Institute, HEALIOS K.K., Kobe, Hyogo, Japan.
Kaoru SasakiResearch Division, Kobe Research Institute, HEALIOS K.K., Kobe, Hyogo, Japan.
Ryosuke KatoriResearch Division, Kobe Research Institute, HEALIOS K.K., Kobe, Hyogo, Japan.
Yasuhiro ShimizuDivision of Matrixome Research and Application, Institute for Protein Research, The University of Osaka, Suita, Osaka, Japan.
Kazumasa FujitaDivision of Matrixome Research and Application, Institute for Protein Research, The University of Osaka, Suita, Osaka, Japan.
Yukimasa TaniguchiDivision of Matrixome Research and Application, Institute for Protein Research, The University of Osaka, Suita, Osaka, Japan.
Taiko KuniedaDivision of Matrixome Research and Application, Institute for Protein Research, The University of Osaka, Suita, Osaka, Japan.
Kouichi TamuraResearch Division, Kobe Research Institute, HEALIOS K.K., Kobe, Hyogo, Japan.
Masashi YamadaResearch Division, Kobe Research Institute, HEALIOS K.K., Kobe, Hyogo, Japan.
Kiyotoshi SekiguchiDivision of Matrixome Research and Application, Institute for Protein Research, The University of Osaka, Suita, Osaka, Japan.
Hironobu KimuraResearch Division, Kobe Research Institute, HEALIOS K.K., Kobe, Hyogo, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human induced pluripotent stem cells (hiPSCs) are a promising source for cell-based and regenerative therapies. In this study, we developed and optimized a chemically defined differentiation protocol to generate hematopoietic progenitor cells (HPCs) from hiPSCs. We demonstrated that basic fibroblast growth factor (bFGF) plays a crucial role in enhancing HPC differentiation, particularly when applied during both the mesoderm (ME) and hematopoietic endothelial (HE) induction stages. Additionally, we explored the use of P-LM421E8, a recombinant fusion protein of laminin421-E8 fragment with domain 1 (D1) of perlecan possessing heparan sulfate (HS) chains. Our findings indicate that P-LM421E8 effectively supports HPC differentiation, generating stable CD34-high/CD117

Indexed as

Basic fibroblast growth factor (bFGF)Chemically defined differentiationHematopoietic progenitor cells (HPCs)Human induced pluripotent stem cells (hiPSCs)Laminin E8 fragmentNatural killer cellsP-LM421E8

Identifiers

PMID41492495
PMCPMC12765145

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.