Evidence map›Paper›PMID 41492301›Full record

ArticleOne health (Amsterdam, Netherlands)2025

A multiplex PCR for rapid detection of vancomycin and linezolid resistance genes in clinical and environmental enterococci: A one health diagnostic approach.

Yusuf Wada, Nabiha A Bouafia, Basim R Al Shammari, Hafeez A Afolabi, Fatimah S M Aljebaly, Mohammed S Almogbel, Mohammed Garout, Muhammad A Halwani, Zainab H Almansour, Ameen S S Alwashmi and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in One health (Amsterdam, Netherlands), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Yusuf WadaDepartment of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Malaysia.
Nabiha A BouafiaInfection prevention and control Centre of Excellence, Prince Sultan Medical Military City, Riyadh 12233, Saudi Arabia.
Basim R Al ShammariDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, University of Hafr Al Batin, Hafr Al Batin 39831, Saudi Arabia.
Hafeez A AfolabiDepartment of General Surgery, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Malaysia.
Fatimah S M AljebalyDepartment of Pathology, College of Medicine, Qassim University, Qassim 56436, Saudi Arabia.
Mohammed S AlmogbelDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Hail, Hail 4030, Saudi Arabia.
Mohammed GaroutDepartment of Community Medicine and Health Care for Pilgrims, Faculty of Medicine, Umm Al-Qura University, Makkah 21955, Saudi Arabia.
Muhammad A HalwaniDepartment of Medical Microbiology, Faculty of Medicine, Al Baha University, Al Baha 4781, Saudi Arabia.
Zainab H AlmansourBiological Science Department, College of Science, King Faisal University, Hofuf 3198, Saudi Arabia.
Ameen S S AlwashmiDepartment of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah 51452, Saudi Arabia.
Nawal A AlKaabiCollege of Medicine and Health Science, Khalifa University, Abu Dhabi 127788, United Arab Emirates.
Maha F Al-SubaieResearch Center, Dr. Sulaiman Alhabib Medical Group, Riyadh 11643, Saudi Arabia.
Ali A RabaanCollege of Medicine, Alfaisal University, Riyadh, Saudi Arabia.
Chan Y YeanDepartment of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Malaysia.
Zaidah Abdul RahmanDepartment of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Malaysia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Antimicrobial resistance (AMR) in Methods: We designed an assay targeting Results: The assay demonstrated high analytical sensitivity, detecting synthetic DNA as low as 1 pg and lysates down to 10 Conclusions: This assay supports integrated AMR surveillance within a One Health framework.

Indexed as

Antimicrobial resistanceClinical microbiologyLinezolid resistanceMultiplex PCROne healthPoultryVancomycin-resistant enterococci

Identifiers

PMID41492301
PMCPMC12765127

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.