ArticleArhiv za higijenu rada i toksikologiju2025
The impact of heat inactivation on RT-qPCR detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2): an experience from the University Clinical Centre of Vojvodina, Serbia
Article in Arhiv za higijenu rada i toksikologiju, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Handling clinical samples from patients suspected of SARS-CoV-2 infection puts healthcare workers at risk of exposure to infectious particles. To reduce this risk, samples are often heat-inactivated before nucleic acid isolation, but this procedure may affect the analytical sensitivity of the test. The aim of this study was therefore to evaluate the effects of heat inactivation (56 °C for 30 min) on RT-qPCR results of samples taken from nasopharyngeal and oropharyngeal (NP/OP) swabs collected from 200 symptomatic patients. Each sample was split into two aliquots - one subjected to heat inactivation and the other stored at 4 °C - followed by nucleic acid isolation and RT-qPCR analysis using the GeneFinder COVID-19 nucleic acid test. Heat inactivation did not significantly affect the overall SARS-CoV-2 detection rate (55.5 % vs. 55.0 % in untreated and heat-treated groups; χ
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