Evidence map›Paper›PMID 41491176›Full record

ArticleBMC veterinary research2026

Protective effect of Lactococcus lactis NZ9000 secreting Bcl2 against Trichinella spiralis infection in mice.

Ruibiao Wang, Suxian Liu, Hongyan Zhang, Doudou Feng, Yukai Lin, Siyang Li, Jiyuan Shen, Bo Wen, Huarun Sun, Minghui Li and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Ruibiao Wang *College of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang, China.
Suxian Liu *College of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang, China.
Hongyan ZhangAffiliated School for Children, Henan Institute of Science and Technology, Xinxiang, China.
Doudou FengCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang, China.
Yukai LinCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang, China.
Siyang LiCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang, China.
Jiyuan ShenCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang, China.
Bo WenCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang, China.
Huarun SunCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang, China.
Minghui LiCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang, China.
Qiangqiang WangCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang, China.
Lan WeiBureau of Agriculture and Rural Affairs of Fengnan District, Tangshan, China.
Yuanfang MaSchool of Basic Medical Sciences, Henan University, Zhengzhou, China.
Yixin LuCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, China. luyixin@neau.edu.cn.
Lei WangCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang, China. wlei_007@163.com.

Funding

Key Research and Development Project of Henan Province 241111110100National Key R&D Program of China 2021YFD1301200National Natural Science Foundation of China 32172862Science and Technology Innovative Research Team in Higher Educational Institutions of Henan Province 24IRTSTHN035The Joint Fund of Science and Technology Research and Development Plan in Henan Province 225200810044the Young Scientists Fund of the Natural Science Foundation of Henan 252300421655
6 · The paper itself

Abstract

backgroundTrichinellosis, a globally distributed zoonosis, threatens both human health and livestock productivity. The limited efficacy of existing anthelmintics underscores the need for innovative strategies to control Trichinella spiralis infection.

resultsA recombinant food-grade Lactococcus lactis strain (NZ-Bcl2) was engineered to secrete the anti-apoptotic protein Bcl2 by fusing the Bcl2 gene to the Usp45 signal peptide. Western blotting confirmed successful expression and secretion. NZ-Bcl2 strains exhibited stable growth, maintained plasmid inheritance over 24 generations without antibiotic pressure, and persisted in the murine intestine for at least seven days. In low-dose infections (150 larvae/mouse), oral administration of NZ-Bcl2 significantly reduced muscle larval burden, mitigated intestinal damage, and alleviated mesenteric lymph node enlargement. These protective effects were associated with downregulation of pro-inflammatory cytokines (IL-1β, TNF-α), upregulation of anti-inflammatory cytokines (IL-10, TGF-β), and increased expression of intestinal barrier markers (claudin-3, MUC-1). Under high-dose challenge (600 larvae/mouse), the protective effects were attenuated.

conclusionRecombinant L. lactis secreting Bcl2 confers partial protection against T. spiralis by enhancing mucosal barrier function and modulating host immunity. These findings highlight the potential of engineered probiotics as a novel platform for antiparasitic intervention, offering translational relevance for food safety and zoonotic disease control.

Indexed as

Lactococcus lactisProto-Oncogene Proteins c-bcl-2Trichinella spiralisTrichinellosisAnimalsCytokinesFemaleLarvaMiceMice, Inbred BALB CCytokinesProto-Oncogene Proteins c-bcl-2Bcl2Gut barrier integrityLactococcus lactisTrichinella spiralis

Identifiers

PMID41491176
PMCPMC12895648

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.