Evidence map›Paper›PMID 41490145›Full record

ArticlePLoS neglected tropical diseases2026

Low risk of transmission of prototype and newly emerged Oropouche virus strains by European Culex pipiens, Aedes albopictus, and Anopheles atroparvus mosquitoes.

Ana Rosales-Rosas, Edith Janssens, Sam Verwimp, Koen Bartholomeeusen, Cédric Van Dun, Katrien Trappeniers, Kevin K Ariën, Ruth Müller, Leen Delang, Marco Brustolin

Abstract read
In one paragraph

Article in PLoS neglected tropical diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ana Rosales-RosasVirus-Host Interactions & Therapeutic Approaches (VITA) Research Group, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium.ORCID https://orcid.org/0000-0002-2099-9152
Edith JanssensVirology Unit, Department of Biomedical Sciences, Institute of Tropical Medicine Antwerp, Antwerp, Belgium.ORCID https://orcid.org/0009-0004-9087-8147
Sam VerwimpVirus-Host Interactions & Therapeutic Approaches (VITA) Research Group, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium.ORCID https://orcid.org/0000-0002-9957-0307
Koen BartholomeeusenVirology Unit, Department of Biomedical Sciences, Institute of Tropical Medicine Antwerp, Antwerp, Belgium.ORCID https://orcid.org/0000-0001-9576-2400
Cédric Van DunVirus-Host Interactions & Therapeutic Approaches (VITA) Research Group, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium.ORCID https://orcid.org/0009-0003-4236-6131
Katrien TrappeniersVirus-Host Interactions & Therapeutic Approaches (VITA) Research Group, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium.ORCID https://orcid.org/0000-0002-8099-6252
Kevin K AriënVirology Unit, Department of Biomedical Sciences, Institute of Tropical Medicine Antwerp, Antwerp, Belgium.ORCID https://orcid.org/0000-0002-1340-4165
Ruth MüllerEntomology Unit, Department of Biomedical Sciences, Institute of Tropical Medicine Antwerp, Antwerp, Belgium.
Leen DelangVirus-Host Interactions & Therapeutic Approaches (VITA) Research Group, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium.ORCID https://orcid.org/0000-0002-8874-675X
Marco BrustolinEntomology Unit, Department of Biomedical Sciences, Institute of Tropical Medicine Antwerp, Antwerp, Belgium.ORCID https://orcid.org/0000-0002-3594-4488

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Oropouche virus (OROV) is an emerging arbovirus of growing public health concern, with increasing incidence and geographic spread. Since its discovery in 1955, OROV has caused multiple outbreaks in South and Central America, with a new introduction in Cuba since May 2024. Recent travel-related cases in Europe and the Americas underscore its potential for global dissemination. Assessing vector competence outside endemic regions is critical in the context of global travel and climate change. We evaluated the vector competence of three mosquito species commonly found in Europe-Culex (Cx.) pipiens, Aedes (Ae.) albopictus, and Anopheles (An.) atroparvus-using two OROV strains: the prototype TRVL9760 (1955, Trinidad and Tobago) and a recent isolate OROV-IRCCS-SCDC_1/2024 (2024, imported from Cuba to Italy). Mosquitoes were orally infected and examined at 7- and 14-days post-infection. We assessed infection (body), dissemination (peripheral tissues), and transmission potential (saliva) by measuring infectious virus particles using the gold standard focus-forming assays. Our findings show that Cx. pipiens and An. atroparvus were not susceptible to infection or did not allow transmission with OROV. In Ae. albopictus, low infection rates were observed: 6.7% of mosquitoes showed infection at day 7 with the prototype strain, and 3.1% at day 14 with OROV-IRCCS-SCDC_1/2024. All infected mosquitoes showed viral dissemination, but none had infectious virus in their saliva, indicating low risk for transmission. These results confirm limited vector competence of European mosquito species for OROV and emphasize the importance of continued entomological surveillance to inform future risk assessments.

Indexed as

AedesAnophelesBunyaviridae InfectionsCulexMosquito VectorsOrthobunyavirusAnimalsCubaEuropeFemaleHumans

Identifiers

PMID41490145
PMCPMC12788654

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.