Evidence map›Paper›PMID 41488317›Full record

ArticleFrontiers in microbiology2025

Rapid visual detection and differentiation of canine and feline parvovirus via a one-tube RPA-CRISPR/Cas13a assay.

Zhurui Shao, Wenkai Zhang, Changbin Jin, Zhibo Yang, Jingyi Yang, Xiaohu Zhang, Xintong Huang, Yufei Yang, Ruizi Ren, Yiwen Zhang and 3 more

Abstract read
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Article in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Zhurui Shao *State Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Wenkai Zhang *State Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Changbin JinState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Zhibo YangState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Jingyi YangState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Xiaohu ZhangState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Xintong HuangState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Yufei YangState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Ruizi RenState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Yiwen ZhangState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Jieen WengState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Yueping ZhangState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Hao ShiState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Canine parvovirus type 2 (CPV-2) and feline parvovirus (FPV) are highly contagious pathogens that pose significant threats to domestic and wild carnivores. Rapid and accurate diagnosis is crucial for outbreak control and wildlife conservation, especially in resource-limited settings. In this study, we developed a one-tube RPA-CRISPR/Cas13a platform for the rapid detection and differentiation of CPV-2 and FPV. Two systems were established: a universal detection system for simultaneous identification of both viruses, and a differential detection system to distinguish between them. By targeting conserved regions of the VP2 gene and optimizing reaction conditions, we achieved high sensitivity and specificity. The universal system exhibited a limit of detection (LOD) of 10

Indexed as

canine parvovirus type 2CRISPR/Cas13afeline parvovirusrapid visual detectionRPAVP2 gene

Identifiers

PMID41488317
PMCPMC12757423

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.