ArticleWorld journal of oncology2026
E2F5 Overexpression in Laryngeal Squamous Cell Carcinoma: Associations With Neutrophil Extracellular Traps in the Tumor Microenvironment.
Article in World journal of oncology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumor of the head and neck, associated with smoking and excessive alcohol consumption. The objective was to investigate the expression pattern of E2F transcription factor 5 (E2F5) in LSCC and its association with neutrophil extracellular traps (NETs), elucidating its role in the tumor microenvironment. Methods: At the cellular level, single-cell RNA sequencing (scRNA-seq) was employed to analyze the expression of E2F5 and NETs-related genes (S100A8, S100A9, LCN2, etc.). At the tissue level, spatial transcriptomics (ST) was used to examine the E2F5 expression pattern. At the mRNA level, E2F5 expression was assessed through mRNA expression profiling, and at the protein level, expression was validated using immunohistochemistry (IHC) on tissue specimens, including 10 LSCC cases (laryngeal, hypopharyngeal, and oropharyngeal squamous cell carcinomas) and 10 non-LSCC controls (benign lesions such as mucoceles, hemangiomas, and polyps). Clustered regularly interspaced short palindromic repeats (CRISPR) knockout screening combined with the CERES algorithm was utilized to evaluate the impact of E2F5 on LSCC cell line proliferation, with negative/positive dependency scores indicating suppression/promotion of growth, respectively. Single-sample Gene Set Enrichment Analysis (ssGSEA) was used to analyze the correlation between E2F5 and immune cells, and chromatin immunoprecipitation sequencing (ChIP-seq) was performed to validate the transcriptional regulation of NETs-related genes by E2F5. Statistical analyses included Wilcoxon, standardized mean difference (SMD), receiver operating characteristic (ROC), and summary receiver operating characteristic (sROC). Results: E2F5 exhibited high expression in LSCC epithelial cells and tissues, with elevated expression at both mRNA and protein levels (SMD = 0.24, 95% confidence interval (CI) = 0.0309 - 0.448, sROC area under the curve (AUC) = 0.71, IHC P = 7.2 × 10 Conclusion: E2F5 is highly expressed in LSCC and is associated with the regulation of NETs-related genes. It may contribute to tumor proliferation and immune evasion by reshaping the tumor microenvironment, highlighting E2F5 as a potential therapeutic target that warrants further functional validation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.