Evidence map›Paper›PMID 41487483›Full record

ArticleFrontiers in veterinary science2025

Isolation and whole-genome characterization of an emerging G9P[23] porcine rotavirus in Central China.

Chaoliang Leng, Jiabao Wang, Jiajing Song, Jiajia Cao, Mengtian Jia, Tianyu Jia, Linyue Xiao, Yingying Zhao, Yu Zhang, Jiayin Zhang and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Chaoliang LengHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology, Henan Province Engineering Research Center of Insect Bioreactor, China-UK International Joint Laboratory for Insect Biology of Henan Province, Nanyang Normal University, Nanyang, China.
Jiabao WangHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology, Henan Province Engineering Research Center of Insect Bioreactor, China-UK International Joint Laboratory for Insect Biology of Henan Province, Nanyang Normal University, Nanyang, China.
Jiajing SongHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology, Henan Province Engineering Research Center of Insect Bioreactor, China-UK International Joint Laboratory for Insect Biology of Henan Province, Nanyang Normal University, Nanyang, China.
Jiajia CaoHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology, Henan Province Engineering Research Center of Insect Bioreactor, China-UK International Joint Laboratory for Insect Biology of Henan Province, Nanyang Normal University, Nanyang, China.
Mengtian JiaHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology, Henan Province Engineering Research Center of Insect Bioreactor, China-UK International Joint Laboratory for Insect Biology of Henan Province, Nanyang Normal University, Nanyang, China.
Tianyu JiaHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology, Henan Province Engineering Research Center of Insect Bioreactor, China-UK International Joint Laboratory for Insect Biology of Henan Province, Nanyang Normal University, Nanyang, China.
Linyue XiaoHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology, Henan Province Engineering Research Center of Insect Bioreactor, China-UK International Joint Laboratory for Insect Biology of Henan Province, Nanyang Normal University, Nanyang, China.
Yingying ZhaoHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology, Henan Province Engineering Research Center of Insect Bioreactor, China-UK International Joint Laboratory for Insect Biology of Henan Province, Nanyang Normal University, Nanyang, China.
Yu ZhangHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology, Henan Province Engineering Research Center of Insect Bioreactor, China-UK International Joint Laboratory for Insect Biology of Henan Province, Nanyang Normal University, Nanyang, China.
Jiayin ZhangHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology, Henan Province Engineering Research Center of Insect Bioreactor, China-UK International Joint Laboratory for Insect Biology of Henan Province, Nanyang Normal University, Nanyang, China.
Hongyue ZhaiHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology, Henan Province Engineering Research Center of Insect Bioreactor, China-UK International Joint Laboratory for Insect Biology of Henan Province, Nanyang Normal University, Nanyang, China.
Na LiHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology, Henan Province Engineering Research Center of Insect Bioreactor, China-UK International Joint Laboratory for Insect Biology of Henan Province, Nanyang Normal University, Nanyang, China.
Hongfei ShiHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology, Henan Province Engineering Research Center of Insect Bioreactor, China-UK International Joint Laboratory for Insect Biology of Henan Province, Nanyang Normal University, Nanyang, China.
Dandan LiHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology, Henan Province Engineering Research Center of Insect Bioreactor, China-UK International Joint Laboratory for Insect Biology of Henan Province, Nanyang Normal University, Nanyang, China.
Yunchao KanHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology, Henan Province Engineering Research Center of Insect Bioreactor, China-UK International Joint Laboratory for Insect Biology of Henan Province, Nanyang Normal University, Nanyang, China.
Lunguang YaoHenan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology, Henan Province Engineering Research Center of Insect Bioreactor, China-UK International Joint Laboratory for Insect Biology of Henan Province, Nanyang Normal University, Nanyang, China.
Zhi-Jun TianState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rotavirus (RV), a significant enteric pathogen, causes diarrheal disease in both nursing piglets and infants. To characterize the genomic features and evolutionary patterns of a porcine rotavirus (PoRV) strain, we isolated viral strains from RT-qPCR confirmed PoRV-positive diarrheic piglet specimens through MA104 cell culture inoculation. Viral isolates were definitively characterized through RT-PCR amplification, indirect immunofluorescence assay (IFA), and transmission electron microscopy (TEM), followed by comprehensive whole-genome sequencing and phylogenetic analysis. The results demonstrated successful isolation of a G9P[23] genotype PoRV, designated HN240916. Whole-genome analysis revealed that the genotype constellation was G9-P[23]-I5-R1-C1-M1-A8-N1-T1-E1-H1. Genomic analysis revealed that the NSP3 and NSP5 genes of the HN240916 strain cluster monophyletically with human RVs, while all remaining genes exhibit close phylogenetic affinity to PoRV lineages. The genotype constellation provides genomic evidence of a past interspecies reassortment event between porcine and human RVs. This study expands PoRV molecular epidemiology resources and enables genotype-based prevention strategies.

Indexed as

evolutionary characterizationG9P[23]isolationporcine rotavirusreassortmentwhole-genome sequencing

Identifiers

PMID41487483
PMCPMC12757227

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.