ArticleACS omega2025
Ligand Valency and Linker Design Dictate the Efficacy of CI-M6PR-Mediated Targeted Delivery of M6P-siRNA Conjugates.
Article in ACS omega, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Targeted delivery of small interfering RNAs (siRNAs) to nonhepatic tissues remains a major challenge in RNAi therapeutics owing to inefficient cellular uptake and off-target effects. In this study, mannose-6-phosphate (M6P)-conjugated siRNAs are developed to target the cation-independent M6P receptor (CI-M6PR/IGF2R), which is overexpressed in various cancers, including chronic myeloid leukemia (CML). Several mono-, di-, and tetravalent M6P-siRNA conjugates are synthesized via solid-phase methods using either flexible hexanediol or rigid proline-based linkers. Thermal melting analysis indicated that M6P conjugation modestly reduced duplex stability, with high-valence constructs and those containing more flexible linkers being particularly affected. By contrast, rigid proline linkers mitigated destabilization. Circular dichroism spectroscopy confirmed that the native A-form RNA structure was maintained after M6P conjugation, while in vitro gene silencing studies in CI-M6PR-positive K562 cells targeting
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Registered trials
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