Heat shock protein 70 in Cynops orientalis: bidirectional thermal regulation and metabolic optimization in amphibian climatic adaptation.

Junrong Liu, Yanqing Tang, Zhengyuan Fang, Zhiwen Wang, Haoyuan Zhang, Songchen Guo, Longhui Lin, Wei Dang

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Article in BMC genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

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1 citing paper in PubMed.

Roles ofInsects · 2026
Article

4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Junrong Liu *Herpetological Research Center, Hangzhou Normal University, Hangzhou, 311121, China.

Yanqing Tang *Herpetological Research Center, Hangzhou Normal University, Hangzhou, 311121, China.

Zhengyuan FangHerpetological Research Center, Hangzhou Normal University, Hangzhou, 311121, China.

Zhiwen WangHerpetological Research Center, Hangzhou Normal University, Hangzhou, 311121, China.

Haoyuan ZhangHerpetological Research Center, Hangzhou Normal University, Hangzhou, 311121, China.

Songchen GuoHerpetological Research Center, Hangzhou Normal University, Hangzhou, 311121, China.

Longhui LinHerpetological Research Center, Hangzhou Normal University, Hangzhou, 311121, China. linlh@hznu.edu.cn.

Wei DangHerpetological Research Center, Hangzhou Normal University, Hangzhou, 311121, China. dangwei@hznu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study investigates the molecular mechanisms underlying thermal resilience in the Cynops orientalis (Chinese fire-bellied newt) through functional characterization of CoHsp70, a cytosolic heat shock protein 70 homolog. Comparative genomic analysis revealed conserved structural domains (ATPase, substrate-binding domain, and EEVD motif) and > 80% sequence identity with amphibian orthologs. Tissue-specific profiling identified fat tissue as the predominant site of CoHsp70 expression. Temperature-dependent transcriptional regulation exhibited bidirectional dynamics: rapid induction under acute heat stress versus progressive suppression during chronic cold exposure. CoHsp70 overexpression enhanced hyperthermic survival while reducing metabolic expenditure (SMR, REE) and oxygen consumption rate (OCR). Taken together, these results suggest that CoHsp70 is a critical modulator of amphibian temperature resilience, balancing proteostatic fidelity with energy conservation under thermal extremes.

Indexed as

Adaptation, PhysiologicalHSP70 Heat-Shock ProteinsSalamandridaeAmino Acid SequenceAnimalsEnergy MetabolismGene Expression RegulationHeat-Shock ResponseOxygen ConsumptionPhylogenyTemperatureHSP70 Heat-Shock ProteinsCoHsp70Cynops orientalisMetabolic efficiencyOverexpressionStress response

Identifiers

PMID41486147

DOI10.1186/s12864-025-12501-0

PMCPMC12870431

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.