Evidence map›Paper›PMID 41486123›Full record

ArticleBMC genomics2026

Transcriptomics identified crucial candidate genes and pathways regulating early testicular development of drakes in the hypothalamic-pituitary-gonadal/thyroid axes.

Yunyi Chen, Xinyue Hu, Xuli Chi, Yifei Lin, Rulong Lin, Hongping Chen, Kaixian Cai, Fenglin Chen, Xiaolong Ye, Xiaopeng Li and 7 more

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Article in BMC genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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17 authors.

Yunyi Chen *State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, P. R. China.
Xinyue Hu *State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, P. R. China.
Xuli ChiState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, P. R. China.
Yifei LinLongyan Longsheng Marketplace Management Group Co., Ltd, Longyan, Fujian, P. R. China.
Rulong LinLongyan Shan-ma Duck Original Breeding Farm, Agricultural Bureau of Xinluo District, Longyan, 364031, PR China.
Hongping ChenLongyan Shan-ma Duck Original Breeding Farm, Agricultural Bureau of Xinluo District, Longyan, 364031, PR China.
Kaixian CaiLongyan Longsheng Marketplace Management Group Co., Ltd, Longyan, Fujian, P. R. China.
Fenglin ChenLongyan Longsheng Marketplace Management Group Co., Ltd, Longyan, Fujian, P. R. China.
Xiaolong YeLongyan Longsheng Marketplace Management Group Co., Ltd, Longyan, Fujian, P. R. China.
Xiaopeng LiState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, P. R. China.
Qingyuan OuyangState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, P. R. China.
Jie LiState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, P. R. China.
Lipeng ZhuState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, P. R. China.
Hehe LiuState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, P. R. China.
Jiwei HuState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, P. R. China.
Liang LiState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, P. R. China.
Jiwen WangState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, P. R. China. wjw2886166@163.com.

Funding

China Agriculture Research System of MOF and MARA CARS-42-4Establishment and Demonstration Extension of Breeding Ability Selection System of Nonghua Drakes 22ZDYFZF0005Key Technology Support Program of Sichuan Province 2021YFYZ0014
6 · The paper itself

Abstract

backgroundThe crosstalk between the hypothalamic-pituitary-gonadal (HPG) and hypothalamic-pituitary-thyroid (HPT) axes plays an important role in gonadal development. However, the mechanism by which these 2 axes regulate early testicular development in drakes remains largely unknown. Therefore, the aim of the present study was to compare the morphology, histology, and transcriptomics between the precocious puberty (PP) and delayed puberty (DP) Longyan Shan-ma drakes (Anas platyrhynchos) to preliminarily reveal the mechanisms regulating early testicular development.

resultThe histomorphological results showed that the colloid area and integrated optical density (IOD) of thyroid in PP group were significantly lower than those in DP group (P < 0.05), and the testis weight, testis index, seminiferous tubule diameter, and number of spermatogonia in PP group were significantly higher than those in DP group (P < 0.05). The transcriptomic results showed that between PP and DP groups, a total of 232, 574, 572, and 4,129 differentially expressed genes (DEGs) were identified in the hypothalamus, pituitary, thyroid, and testis, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that DEGs in hypothalamus, pituitary, thyroid, and testis were significantly enriched in 3, 14, 16, and 27 KEGG pathways (P < 0.05), respectively, with the neuroactive ligand-receptor interaction pathway being the only one commonly enriched in all 4 tissues. Further, 11 key genes (GNRH1, AGT, AGTR1, COL1A1, ITGB6, PIK3CB, PLCB4, CALML4, ADCY1, PDE1C, and BUB1) were identified through screening based on enrichment in core reproductive pathways (including neuroactive ligand-receptor interaction and progesterone-mediated oocyte maturation) and functional links to testicular development. Their expression trends were validated by qRT-PCR (R²=0.89), confirming reliability. These genes may serve as critical nodes in HPG/HPT axis crosstalk to regulate early testicular development.

conclusionThis study is the first systematically comparing the transcriptomes of the HPG and HPT axes between DP and PP drakes. These results will provide novel insights into the mechanisms of crosstalk regulation of the HPG and HPT axes on early testicular development in drakes.

Indexed as

Gene Expression ProfilingHypothalamo-Hypophyseal SystemTestisThyroid GlandTranscriptomeAnimalsHypothalamusMaleDrakeEarly testicular developmentHPG axisHPT axisTranscriptome

Identifiers

PMID41486123
PMCPMC12870974

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.