ReviewCurrent opinion in microbiology2026
Model-guided design of regulatable promoters for synthetic biology.
Review in Current opinion in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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2 authors.
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Abstract
Designing regulatable promoters with specified functional output remains difficult because natural promoters are unlikely to match a particular specification, and the sequence design space is large, complex, and challenging to interpret. This review advances a context-minimized, measurement-first approach in Escherichia coli that couples simple assays to a single transcription factor (TF)-based thermodynamic framework. The model is structured around two key concepts related to the TF: occupancy and function. Here, we outline how these concepts can be manipulated and measured at the level of DNA sequence and how those perturbations can impact fold-change and thus features of the promoter, such as dynamic range, leakiness, and sensitivity. LacI serves as a worked example in which sequence-occupancy, copy number, and competition, position-dependent function, and inducer allostery have been measured and can be combined to optimize response features. Overall, simple measurements linked to interpretable models provide a practical route to compiling desired regulatory specifications into sequence-level designs.
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