Evidence map›Paper›PMID 41484744›Full record

ArticleApplied biochemistry and biotechnology2026

Construction of Engineered EcN Bacteria Constitutively Highly Expressing RtSOD/RtSOD-CAT Fusion Protein and its Protective Effect on UC Mice.

Zhuo Jiang, Qi Lin, Jiamin Zhu, Shiwei Qing, Zhan Luo, Shang Lv, Xuebing Yang, Dan Wang, Jianqing Chen, Zhengbing Lv and 1 more

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Article in Applied biochemistry and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Zhuo Jiang *College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, 310018, China.
Qi Lin *College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, 310018, China.
Jiamin Zhu *Zhejiang Sci-Tech University Shaoxing Academy of Biomedicine, Shaoxing, Zhejiang, 312369, China.
Shiwei QingCollege of Foreign Language, Guangdong Pharmaceutical University, Guangzhou, Guangdong, 510006, China.
Zhan LuoCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, 310018, China.
Shang LvMarine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, China.
Xuebing YangCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, 310018, China.
Dan WangCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, 310018, China.
Jianqing ChenCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, 310018, China.
Zhengbing LvCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, 310018, China.
Zuoming NieCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, 310018, China. wuxinzm@zstu.edu.cn.ORCID http://orcid.org/0000-0002-0464-2241

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Superoxide dismutase (SOD) is an antioxidant enzyme containing different metal ion, which can reduce oxidative stress and treat ulcerative colitis (UC). In addition, SOD's therapeutic effect can be enhanced when combined with other antioxidant enzymes, such as catalase (CAT). In this study, the probiotic Escherichia coli Nissle 1917 (EcN) was used as a chassis strain to develop two engineered variants, EcNc-RtSOD and EcNc-MLH. By using two cryptic plasmids, these strains were modified to constitutively express RtSOD and RtSOD-CAT (MLH), respectively. Furthermore, the engineered EcN strains were found to significantly reduce DSS-induced intestinal inflammation and promote intestinal mucosal healing in UC mice.Biochemical analyses revealed that the levels of superoxide dismutase (SOD) and catalase (CAT) in intestinal tissues were significantly elevated, while malondialdehyde (MDA) content was markedly reduced. Compared with the DSS group, the expression level of interleukin-6 (IL-6) in colon tissues of mice treated with RtSOD and MLH decreased by 24.04% and 37.68%, respectively. Similarly, the expression level of interleukin-1β (IL-1β) was reduced by 14.22% and 28.73%, respectively, in the RtSOD and MLH treatment groups compared to the DSS group. This study proposes a novel strategy for UC treatment that utilizes therapeutically engineered bacterial strains.

Indexed as

CatalaseColitis, UlcerativeEscherichia coliRecombinant Fusion ProteinsSuperoxide DismutaseAnimalsInterleukin-6MaleMiceCatalaseInterleukin-6Recombinant Fusion ProteinsSuperoxide DismutaseAnti-inflammatoryCatalase (CAT)EcN engineered strainSuperoxide dismutase (SOD)Ulcerative colitis (UC)

Identifiers

PMID41484744

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.