ArticleGenome biology2026
Unravelling the progression of the zebrafish primary body axis with reconstructed spatiotemporal transcriptomics.
Article in Genome biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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13 authors.
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Abstract
backgroundElucidating the spatiotemporal dynamics of gene expression is essential for understanding complex physiological and pathological processes. Current spatial transcriptomics techniques are hindered by low read depths and limited gene detection.
resultsHere, we introduce Palette, a pipeline that infers detailed spatial gene expression patterns from bulk RNA-seq data, utilizing existing spatial transcriptomics data as the sole reference. This method identifies more precise expression patterns by smoothing, imputing and adjusting gene expressions. We apply Palette to reconstruct the zebrafish SpatioTemporal Expression Profiles (zSTEP) by integrating 53-slice serial bulk RNA-seq data from three developmental stages with existing spatial transcriptomics and image references. zSTEP provides a comprehensive cartographic resource for examining gene expression and investigating developmental events within zebrafish embryos. Utilizing machine learning-based screening, we identify key morphogens and transcription factors essential for anteroposterior axis development and characterized their dynamic distribution throughout embryogenesis. In addition, among these transcription factors, Hox family genes are found to be pivotal in anteroposterior axis refinement. Their expression is closely correlated with cellular anteroposterior identities, and hoxb genes may act as central regulators in this process.
conclusionsThis study presents Palette, a pipeline for integrating bulk RNA-seq data and spatial transcriptomics data, and zSTEP, a comprehensive cartographic resource for investigating zebrafish early embryonic development. In addition, key morphogens and transcriptional factors essential for anteroposterior axis establishment and refinement are identified.
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