Evidence map›Paper›PMID 41484455›Full record

ArticleCancer immunology, immunotherapy : CII2026

Pseudomonas aeruginosa induces tumor pyroptosis and immune activation to enhance checkpoint blockade in colorectal cancer.

Yanan Hu, Rong Li, Jiaxi Feng, Ningyi Sun, Wei Zheng, Xin Jin, Guodong Wang, Yi He, Yiwei Hao, Jun Zhang and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer immunology, immunotherapy : CII, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Yanan Hu *The Fourth Affiliated Hospital of Nanjing Medical University, Nanjing, 210032, China.
Rong Li *Department of Neurology, Xindu District People's Hospital of Chengdu, Chengdu, 610500, China.
Jiaxi Feng *The Fourth Affiliated Hospital of Nanjing Medical University, Nanjing, 210032, China.
Ningyi SunDepartment of Clinical Microbiology, School of Laboratory Medicine, Chengdu Medical College, Clinical IVD Joint Research Center of Chengdu Medical College-Maccura Biotechnology, Chengdu, 610500, China.
Wei ZhengThe Fourth Affiliated Hospital of Nanjing Medical University, Nanjing, 210032, China.
Xin JinThe Fourth Affiliated Hospital of Nanjing Medical University, Nanjing, 210032, China.
Guodong WangDepartment of Clinical Microbiology, School of Laboratory Medicine, Chengdu Medical College, Clinical IVD Joint Research Center of Chengdu Medical College-Maccura Biotechnology, Chengdu, 610500, China.
Yi HeDepartment of Clinical Microbiology, School of Laboratory Medicine, Chengdu Medical College, Clinical IVD Joint Research Center of Chengdu Medical College-Maccura Biotechnology, Chengdu, 610500, China.
Yiwei HaoTaishan Community Health Service Center, Jiangbei New District, Nanjing, 210032, China.
Jun ZhangDepartment of Neurology, Xindu District People's Hospital of Chengdu, Chengdu, 610500, China. 501838695@qq.com.
Ke RenDepartment of Clinical Microbiology, School of Laboratory Medicine, Chengdu Medical College, Clinical IVD Joint Research Center of Chengdu Medical College-Maccura Biotechnology, Chengdu, 610500, China. renke@cmc.edu.cn.
Xin WuThe Fourth Affiliated Hospital of Nanjing Medical University, Nanjing, 210032, China. 2608236988@qq.com.

Funding

Nanjing Municipal Health Science and Technology Development Project YKK22214The Clinical Science Research Fund of Chengdu Medical College Chengdu Xindu District People's Hospital 2022LHXD-02The CMC Excellent-talent Program Foundation 2024qnGzn13the Sichuan Administration of Traditional Chinese Medicine 2024MS149the Sichuan Science and Technology Program 2025NSFSC2139
6 · The paper itself

Abstract

Colorectal cancer (CRC) exhibits limited responsiveness to immune checkpoint inhibitors (ICIs), largely due to its immunosuppressive tumor microenvironment (TME) and poor baseline immunogenicity. Here, we report that Pseudomonas aeruginosa (P. aeruginosa) triggers caspase-3-dependent pyroptosis in murine CRC MC38 cells, characterized by GSDME cleavage, intracellular reactive oxygen species (ROS) accumulation, and the release of damage-associated molecular patterns (DAMPs). This form of immunogenic cell death promotes robust inflammatory cytokine secretion, upregulation of PD-L1 on tumor cells, and functional maturation of bone marrow-derived dendritic cells (BMDCs) in vitro. In vivo, intratumoral injection of P. aeruginosa leads to significant reprogramming of the TME, including increased expression of proinflammatory genes, DC maturation, and enhanced infiltration of CD8

Indexed as

Colorectal NeoplasmsImmune Checkpoint InhibitorsPseudomonas aeruginosaPseudomonas InfectionsPyroptosisAnimalsB7-H1 AntigenCell Line, TumorDendritic CellsFemaleHumansMiceMice, Inbred C57BLTumor MicroenvironmentB7-H1 AntigenImmune Checkpoint InhibitorsColorectal cancerImmune checkpoint blockadePseudomonas aeruginosaPyroptosisTumor immune microenvironment

Identifiers

PMID41484455
PMCPMC12764741

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.