An insulin receptor activity surge in follicle cells drives vitellogenesis by upregulating CrebA.

Xiaoya Wang, Huanju Liu, Zhiyong Yin, Tianning Shao, Lin Li, Jun Ma, Feng He

Abstract read

In one paragraph

Article in EMBO reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it

0cells of the map it votes in

2citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

Targeting the 20E-responsive miR-2788-Treh1 axis disrupts chitin biosynthesis and ovarian development in the grassland pest Galeruca daurica.Pest management science · 2026
Article
Credit to the Fruit Fly: How the Tiny Insect Lights Up Our Understanding of Human Disease.Insects · 2026
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Xiaoya Wang *Department of Obstetrics and Gynecology, the Fourth Affiliated Hospital of School of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, China.ORCID 0009-0007-8725-0473

Huanju Liu *Institute of Genetics, International School of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, China.ORCID 0000-0002-3370-4296

Zhiyong YinInstitute of Genetics, International School of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, China.

Tianning ShaoDepartment of Obstetrics and Gynecology, the Fourth Affiliated Hospital of School of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, China.

Lin LiDepartment of Obstetrics and Gynecology, the Fourth Affiliated Hospital of School of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, China.

Jun MaDepartment of Obstetrics and Gynecology, the Fourth Affiliated Hospital of School of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, China. jun_ma@zju.edu.cn.ORCID 0000-0002-1609-3294

Feng HeDepartment of Obstetrics and Gynecology, the Fourth Affiliated Hospital of School of Medicine, Zhejiang University, Yiwu, Zhejiang, 322000, China. feng_he@zju.edu.cn.ORCID 0000-0001-7114-8078

Funding

MOST | National Key Research and Development Program of China (NKPs) 2021YFC2700403MOST | National Natural Science Foundation of China (NSFC) 32470584

6 · The paper itself

Abstract

Folliculogenesis is a process that requires accurate interpretation of female physiological cues and elaborate coordination between the growing oocyte and its surrounding follicle cells, each being capable of responding to external signals. Here, we investigate the role of insulin signaling in Drosophila follicle cells. Using a phase separation-based reporter system, we observe a surge of insulin receptor activity in follicle cells during vitellogenic stages, a surge that is disrupted by a maternal high-sucrose diet. Single-cell RNA-seq reveals a diet-sensitive subpopulation of stage-8 follicle cells, which exhibits a reduction in CrebA-mediated transcription of genes for yolk and vitelline membrane proteins. Our results suggest a critical role of CrebA in implementing the stage-specific effect of insulin signaling to boost the secretory capacity of follicle cells. Mechanistically, CrebA is directly repressed by nuclear FoxO that is subject to insulin control, a regulatory axis that we show is conserved in human granulosa cells. This study delineates a mechanism through which insulin and nutrient cues act on a developmental transition via modulating the biosynthetic and secretory functions of the ovary.

Indexed as

Cyclic AMP Response Element-Binding ProteinDrosophila ProteinsOvarian FollicleReceptor, InsulinVitellogenesisAnimalsDrosophila melanogasterFemaleForkhead Transcription FactorsGene Expression Regulation, DevelopmentalGranulosa CellsHumansInsulinOocytesSignal TransductionUp-RegulationCyclic AMP Response Element-Binding ProteinDrosophila ProteinsForkhead Transcription FactorsInsulinReceptor, InsulinCyclic-AMP Response Element Binding Transcription FactorFolliculogenesisHigh-Sucrose DietInsulin

Identifiers

PMID41484377

DOI10.1038/s44319-025-00672-6

PMCPMC12894986

What OpenQuestion holds

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LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.