Evidence map›Paper›PMID 41483224›Full record

ArticlePlanta2026

Inhibition of photosystems and respiratory complexes enhances lipid biosynthesis via ROS in Chlorella pyrenoidosa SHOU-1002 under nitrogen limitation.

Liufu Wang, Xueyan Ma, Xuxiong Huang, Haibo Wen, Wu Jin, Wanwen Chen, Yufeng Wang, Pao Xu, Hui Yang, Yingying Zhang

Abstract read
PubMed Publisher
In one paragraph

Article in Planta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Liufu Wang *Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, No. 9, Shanshui East Road, Binhu District, Wuxi, 214081, China.
Xueyan Ma *Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, No. 9, Shanshui East Road, Binhu District, Wuxi, 214081, China.
Xuxiong HuangCentre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China. 1103173569@qq.com.ORCID http://orcid.org/0000-0002-0344-9319
Haibo WenKey Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, No. 9, Shanshui East Road, Binhu District, Wuxi, 214081, China. Wenhaibo@163.com.
Wu JinKey Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, No. 9, Shanshui East Road, Binhu District, Wuxi, 214081, China.
Wanwen ChenKey Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, No. 9, Shanshui East Road, Binhu District, Wuxi, 214081, China.
Yufeng WangKey Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, No. 9, Shanshui East Road, Binhu District, Wuxi, 214081, China.
Pao XuKey Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, No. 9, Shanshui East Road, Binhu District, Wuxi, 214081, China.
Hui YangCollege of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
Yingying ZhangCollege of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.

Funding

Central Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences 2025JBFR06Natural Science Foundation of Jilin Province 31800435
6 · The paper itself

Abstract

MAIN

conclusionThe suppression of photosystem II, photosystem I, respiratory complex I, and respiratory complex III triggers lipid biosynthesis via increased ROS in Chlorella pyrenoidosa SHOU-1002 under nitrogen limitation. This study investigated the regulation of photosynthesis and respiration in lipid biosynthesis. Chlorella pyrenoidosa SHOU-1002 was analyzed using biochemical and molecular biological approaches under nitrogen limitation, chemical treatments, and RNA interference (RNAi). The results showed that nitrogen limitation redirected carbon flux from chlorophyll, carbohydrates, and proteins to lipids, yielding biodiesel-suitable lipids with a calculated degree of unsaturation ≤ 92.80%. Mechanistically, nitrogen limitation inhibited the activities of photosystem II (PSII), photosystem I (PSI), respiratory complex I (RCI), and respiratory complex III (RCIII) by downregulating the expression of their constituent genes. This suppression led to increased levels of reactive oxygen species (ROS) and subsequent lipid accumulation. RNAi of these complexes similarly enhanced ROS and lipid accumulation. These results support a conclusion that photosynthetic and respiratory inhibition drives ROS-mediated lipid accumulation in C. pyrenoidosa SHOU-1002. This finding enhances our understanding of microalgal lipid biosynthesis under nitrogen limitation and could contribute to the development of the microalgae-biofuel industry through metabolic engineering.

Indexed as

ChlorellaLipidsNitrogenReactive Oxygen SpeciesChlorophyllLipid MetabolismPhotosynthesisPhotosystem II Protein ComplexPhotosystem I Protein ComplexRNA InterferenceChlorophyllLipidsNitrogenPhotosystem II Protein ComplexPhotosystem I Protein ComplexReactive Oxygen SpeciesBiofuelLipid accumulationMicroalgaePhotosystemsRespiratory complexes

Identifiers

PMID41483224

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.